Membrane cell grafts , fresh and frozen, to cover full thickness wounds in athymic nude mice

Membrane cell grafts , fresh and frozen, to cover full thickness wounds in athymic nude mice Biobrane, an adherent, flexible temporary wound dressing was incubated with cultured human keratinocytes. The cells adhered quickly, forming ”membrane-cell-grafts” (MCG). Some of the grafts were frozen and, after thawing, viability was verified with an XTT colorimetric assay. MCGs, fresh and cryopreserved, were transplanted on full thickness wounds created on athymic nude mice and resulted in a differentiated epithelium of human phenotype. For further investigation immunohistochemistry, immunofluorescence and electronmicroscopy were performed. Conventional cultured epidermal grafts (CEG) and wounds without cell grafts served as control. Compared with CEG-grafted sites a reduced wound contraction was noticed and complete remodelling of the basement membrane zone was found. The effectiveness of the easy, uncomplicated production, cryopreservation and use, as well as the short culture period could lead to a new approach in the treatment of burns and chronic wounds. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Plastic Surgery Springer Journals

Membrane cell grafts , fresh and frozen, to cover full thickness wounds in athymic nude mice

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Publisher
Springer Journals
Copyright
Copyright © 1999 by Springer-Verlag Berlin Heidelberg
Subject
Medicine & Public Health; Plastic Surgery
ISSN
0930-343X
eISSN
1435-0130
D.O.I.
10.1007/s002380050191
Publisher site
See Article on Publisher Site

Abstract

Biobrane, an adherent, flexible temporary wound dressing was incubated with cultured human keratinocytes. The cells adhered quickly, forming ”membrane-cell-grafts” (MCG). Some of the grafts were frozen and, after thawing, viability was verified with an XTT colorimetric assay. MCGs, fresh and cryopreserved, were transplanted on full thickness wounds created on athymic nude mice and resulted in a differentiated epithelium of human phenotype. For further investigation immunohistochemistry, immunofluorescence and electronmicroscopy were performed. Conventional cultured epidermal grafts (CEG) and wounds without cell grafts served as control. Compared with CEG-grafted sites a reduced wound contraction was noticed and complete remodelling of the basement membrane zone was found. The effectiveness of the easy, uncomplicated production, cryopreservation and use, as well as the short culture period could lead to a new approach in the treatment of burns and chronic wounds.

Journal

European Journal of Plastic SurgerySpringer Journals

Published: Aug 16, 1999

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