Melanin-Mediated Synthesis of Silver Nanoparticles and Their Affinity Towards Tyrosinase

Melanin-Mediated Synthesis of Silver Nanoparticles and Their Affinity Towards Tyrosinase The bacterial strain Pseudomonas sp. SSA has capacity to produce extracellular melanin that sequesters heavy metals. The brown-black melanin pigment was observed in the culture liquid and mediated synthesis of silver nanoparticles (AgNPs). The AgNPs were characterized using UV–visible, dynamic light scattering, energy dispersive X-ray, Fourier transform infrared and surface plasmon resonance spectroscopy, scanning electron and transmission electron microscopy and selected area electron diffraction analysis. The synthesized nanoparticles were found to be spherical in shape with size in the range of 14–30 nm and showed high antimicrobial activity against pathogenic bacteria and fungi. These nanoparticles revealed binding affinity towards fungal and human tyrosinases with K D 4.601 × 10–10 and 2.816 × 10–5 M, respectively. In addition, produced nanoparticles did not show any toxic effect towards HeLa cells up to 20 μg/mL. These nanoparticles could find application in medicine and cosmetics due to their enzyme inhibition and antimicrobial activities. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Melanin-Mediated Synthesis of Silver Nanoparticles and Their Affinity Towards Tyrosinase

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Publisher
Pleiades Publishing
Copyright
Copyright © 2018 by Pleiades Publishing, Inc.
Subject
Life Sciences; Biochemistry, general; Microbiology; Medical Microbiology
ISSN
0003-6838
eISSN
1608-3024
D.O.I.
10.1134/S0003683818020096
Publisher site
See Article on Publisher Site

Abstract

The bacterial strain Pseudomonas sp. SSA has capacity to produce extracellular melanin that sequesters heavy metals. The brown-black melanin pigment was observed in the culture liquid and mediated synthesis of silver nanoparticles (AgNPs). The AgNPs were characterized using UV–visible, dynamic light scattering, energy dispersive X-ray, Fourier transform infrared and surface plasmon resonance spectroscopy, scanning electron and transmission electron microscopy and selected area electron diffraction analysis. The synthesized nanoparticles were found to be spherical in shape with size in the range of 14–30 nm and showed high antimicrobial activity against pathogenic bacteria and fungi. These nanoparticles revealed binding affinity towards fungal and human tyrosinases with K D 4.601 × 10–10 and 2.816 × 10–5 M, respectively. In addition, produced nanoparticles did not show any toxic effect towards HeLa cells up to 20 μg/mL. These nanoparticles could find application in medicine and cosmetics due to their enzyme inhibition and antimicrobial activities.

Journal

Applied Biochemistry and MicrobiologySpringer Journals

Published: Mar 13, 2018

References

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