Melandrium yellow fleck bromovirus (MYFV) systemically infected Arabidopsis thaliana , although the susceptibility of several A. thaliana accessions to MYFV differed from their susceptibility to the other two bromoviruses infecting A. thaliana . We constructed full-length cDNA clones of MYFV genomic RNAs 1, 2, and 3 and determined their complete nucleotide sequences. Similar to Broad bean mottle bromovirus , (1) the 5′-terminal nucleotide of the MYFV genomic RNAs was adenine, and (2) the “D-arm” was absent from the tRNA-like structure in the 3′ untranslated regions (UTRs) of MYFV RNAs. As unique characteristics, MYFV RNA3 lacked the poly(A) tract in the intercistronic region and contained a directly repeated sequence of about 200 nucleotides and polypyrimidine tracts of heterogeneous lengths in the 5′ UTR. Co-infection experiments using RNA3 clones with or without the duplicated sequence demonstrated that the duplication contributed to the competitive fitness of the virus in Nicotiana benthamiana .
Archives of Virology – Springer Journals
Published: Sep 1, 2009
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