Mefloquine Effects on the Lens Suggest Cooperative Gating of Gap Junction Channels

Mefloquine Effects on the Lens Suggest Cooperative Gating of Gap Junction Channels Mefloquine (MFQ) selectively blocks exogenously expressed gap junction channels composed of C×50 but not C×46. The purpose of the current study was to evaluate MFQ effects on wild-type (WT) mouse lenses that express both C×50 and C×46 in their outer shell of differentiating fibers (DFs). Lenses in which C×46 was knocked into both C×50 alleles (KI) were used as controls; MFQ had no effect on coupling in these lenses. When WT lenses were exposed to MFQ, the DF coupling conductance decreased significantly, suggesting that C×50 contributes about 57% of the coupling conductance in DF and C×46 contributes 43%. Remarkably, in the presence of MFQ, the 43% of the channels that remained open did not gate closed in response to a reduction in pH, whereas in the absence of MFQ, the same pH change caused all the DF channels to gate closed. Since MFQ is a selective blocker of C×50 channels, it appears that C×46 channels lack pH-mediated gating in the absence of functional C×50 channels but are pH-sensitive in the presence of C×50 channels. These results suggest the two types of channels interact and gate cooperatively. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Mefloquine Effects on the Lens Suggest Cooperative Gating of Gap Junction Channels

Loading next page...
 
/lp/springer_journal/mefloquine-effects-on-the-lens-suggest-cooperative-gating-of-gap-0zsGFR6Mv4
Publisher
Springer-Verlag
Copyright
Copyright © 2006 by Springer Science+Business Media, Inc.
Subject
Life Sciences; Human Physiology; Biochemistry, general
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s00232-006-0021-6
Publisher site
See Article on Publisher Site

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial