GeroScience https://doi.org/10.1007/s11357-018-0021-3 METHODS Measurement of respiratory function in isolated cardiac mitochondria using Seahorse XFe24 Analyzer: applications for aging research Siva S. V. P. Sakamuri & Jared A. Sperling & Venkata N. Sure & Monica H. Dholakia & Nicholas R. Peterson & Ibolya Rutkai & Padmini S. Mahalingam & Ryosuke Satou & Prasad V. G. Katakam Received: 6 April 2018 /Accepted: 30 April 2018 American Aging Association 2018 Abstract Mitochondria play a critical role in the car- Keywords Cardiac mitochondria Respiratory control diomyocyte physiology by generating majority of the ratio Oxygen consumption rate ATP required for the contraction/relaxation through ox- idative phosphorylation (OXPHOS). Aging is a major risk factor for cardiovascular diseases (CVD) and mito- Introduction chondrial dysfunction has been proposed as potential cause of aging. Recent technological innovations in Mitochondria, the power houses of the cells, generate Seahorse XFe24 Analyzer enhanced the detection sen- ATP through oxidation of NADH and FADH . Transfer sitivity of oxygen consumption rate and proton flux to of electrons from NADH to the oxygen through electron advance our ability study mitochondrial function. Stud- transport chain (ETC) is coupled with proton transfer ies of the respiratory function tests in the isolated mito- across the inner
AGE – Springer Journals
Published: Jun 2, 2018
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