Mammalian Genome 8,153-156 (1997). *~rel lome 9 Sprlnger-Verlag New York lnc 1997 Mass production of genetic markers from a limited number of sorted chromosomes D. Vaiman, 1 E. Paiihoux, z A. Schmitz, 3 C. Giraud-Delville, 1 C. Cotinot, 2 E.P. Cribiu I JlNRA, Drpartement de Grnrhque Animale, Laboratoire de Grn&ique Biochlmique et de Cytogrnrtique, F-78352 Jouy-en-Josas, France 2Laboratolre de Biologie Cellulaire et Molrculaire, F-78352 Jouy-en-Josas, France 3CEA, Direction des Sciences du vlvant, Laboratolre de Cytomrtrie, BP6, F-92265 Fontenay-aux-Roses, France Received: 22 July 1996 / Accepted: 19 September 1996 Low-resolution maps composed mainly of microsatellites have al- flow cytometer (Becton Dickinson) equipped with two coherent ready been established in cattle (Barendse et al. 1994; Bishop et al, lasers 306 and 307 (Palo Alto, Calif., USA). The primary laser was 1994), pigs (Archibald et al. 1995; Rohrer et al. 1994), sheep tuned to excite the Hoechst 33258 in the UV (358-361 nm, 0.5 W) (Crawford et al. 1995), and goats (Vaiman et al. 1996b). These and the secondary laser was tuned to 458 nm to excite chromo- maps have already proven their efficiency for locating Mendelian mycin A3 (0.5 W). The Hoechst 33258 fluorescence was collected genes (Cockett et al. 1994;
Mammalian Genome – Springer Journals
Published: Mar 24, 2009
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