Mass production of genetic markers from a limited number of sorted chromosomes

Mass production of genetic markers from a limited number of sorted chromosomes Mammalian Genome 8,153-156 (1997). *~rel lome 9 Sprlnger-Verlag New York lnc 1997 Mass production of genetic markers from a limited number of sorted chromosomes D. Vaiman, 1 E. Paiihoux, z A. Schmitz, 3 C. Giraud-Delville, 1 C. Cotinot, 2 E.P. Cribiu I JlNRA, Drpartement de Grnrhque Animale, Laboratoire de Grn&ique Biochlmique et de Cytogrnrtique, F-78352 Jouy-en-Josas, France 2Laboratolre de Biologie Cellulaire et Molrculaire, F-78352 Jouy-en-Josas, France 3CEA, Direction des Sciences du vlvant, Laboratolre de Cytomrtrie, BP6, F-92265 Fontenay-aux-Roses, France Received: 22 July 1996 / Accepted: 19 September 1996 Low-resolution maps composed mainly of microsatellites have al- flow cytometer (Becton Dickinson) equipped with two coherent ready been established in cattle (Barendse et al. 1994; Bishop et al, lasers 306 and 307 (Palo Alto, Calif., USA). The primary laser was 1994), pigs (Archibald et al. 1995; Rohrer et al. 1994), sheep tuned to excite the Hoechst 33258 in the UV (358-361 nm, 0.5 W) (Crawford et al. 1995), and goats (Vaiman et al. 1996b). These and the secondary laser was tuned to 458 nm to excite chromo- maps have already proven their efficiency for locating Mendelian mycin A3 (0.5 W). The Hoechst 33258 fluorescence was collected genes (Cockett et al. 1994; Mammalian Genome Springer Journals

Mass production of genetic markers from a limited number of sorted chromosomes

Loading next page...
Copyright © 1997 by Springer-Verlag
Life Sciences; Cell Biology; Anatomy; Zoology
Publisher site
See Article on Publisher Site


You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial