Mammalian Genome 8, Brief Data Reports 377 Locus symbol: Sycp3 1). This localization was subsequently confirmed by using a Chr Map position: Chromosome (Chr) 10, band C 10 paint probe from Cambio (Cambridge, UK) that painted only Method of mapping: Fluorescent in situ hybridization, chromo- the chromosomes showing a Sycp3 FISH signal (data not shown). some painting No mouse mutant with a phenotype affecting reproductive organs Database deposit information: EMBL accession nos. Y08485, or fertility has been described in this region. Y08486 To show that the detected locus on Chr 10 is an expressed Molecular reagents: Primers for isolating mouse Sycp3: (+), 5'- locus rather than, for instance, a processed pseudogene, a 6.7-kb GGTGGAAGAAAGCAT/CTCTGG-3'; (-), 5'-CAGCTC- EcoRI subfragment of SCP314 containing the 3' untranslated re- CAAATTTTTCCAGC-3'. gion of Sycp3 was subcloned and sequence analysis performed Primers for sequencing from phage clone (SCP3/4): (+), 5'- with two primers from that region. An intron/exon junction was TTTGAAGAAAGAACTTGAACC-3'; (-), 5i-CCTGAAAGTTT- found at a site corresponding to nt 800 of the Sycp3 cDNA, 51 nt GATACATTGG-3f = '. upstream of the stop codons, ruling out the presence of an intron- Previously identified homologs: The SCP3 genes in rat and ham- less pseudogene. ster
Mammalian Genome – Springer Journals
Published: Mar 21, 2009
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