Banana bunchy top virus (BBTV), a multi-component circular ssDNA virus, replicates via a dsDNA intermediate that also serves as a template for virion sense transcription. Seven virus-derived transcripts have been previously identified and analysed in BBTV-infected bananas by northern analysis and 3′ rapid amplification of cDNA ends (3′ RACE). In this study, we have used RNA ligase-mediated rapid amplification of 5′ cDNA ends (RLM-RACE) to complete the mapping of the BBTV gene transcripts and have now fully mapped the transcribed regions of each BBTV component and effectively defined the upstream regulatory region.
Archives of Virology – Springer Journals
Published: Mar 1, 2007
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