Mapping the 5′ ends of banana bunchy top virus gene transcripts

Mapping the 5′ ends of banana bunchy top virus gene transcripts Banana bunchy top virus (BBTV), a multi-component circular ssDNA virus, replicates via a dsDNA intermediate that also serves as a template for virion sense transcription. Seven virus-derived transcripts have been previously identified and analysed in BBTV-infected bananas by northern analysis and 3′ rapid amplification of cDNA ends (3′ RACE). In this study, we have used RNA ligase-mediated rapid amplification of 5′ cDNA ends (RLM-RACE) to complete the mapping of the BBTV gene transcripts and have now fully mapped the transcribed regions of each BBTV component and effectively defined the upstream regulatory region. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Mapping the 5′ ends of banana bunchy top virus gene transcripts

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Publisher
Springer-Verlag
Copyright
Copyright © 2007 by Springer-Verlag
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-006-0889-6
Publisher site
See Article on Publisher Site

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