Mapping of the grt locus to mouse Chromosome 5

Mapping of the grt locus to mouse Chromosome 5 944 Mammalian Genome 8, Brief Data Reports 9. Wang N, Weng W, Breslow JL, Tall AR (1996) J Biol Chem 271, 21001-21004 10. Purcell-Huynh DA, Weinreb A, Castellani LW, Mehrabian M, Doolittle MH, Lusis AJ (1995) J Clin Invest 96, 1845-1858 05Mit254 ms nm m 11. Machleder D, Ivandic B, Welch C, Castellani L, Reue K, Lusis AJ grt (1997) J Clin Invest 99, 1406-1419 m msu n D5Mit240 msm msm Mapping of the grt locus to mouse D5Mit314 Chromosome 5 D5Mit338 msmsm m D5Mit29 T. Agui, 1 T. Miyamoto, 1 H. Tsumura, z T. Yoshida 3 52 49 11 14 12 10 1 1 qnstitute for Experimental Animal Science, Nagoya City University Medical School, Nagoya, Aichi 467, Japan B 2Institute of Laboratory Animals, Mie University School of Medicine, Chr 5 Tsu, Mie 514, Japan 3yoshida Institute for General Research of Life, Suginami, Tokyo 168, Japan Received: 13 May 1997 / Accepted: 8 August 1997 Species: Mouse DSMit254 Locus name: growth-retarded Locus symbol: grt 18.( Map position: The grt is localized on mouse Chromosome (Chr) 5, 59 cM distal from the centromere: centromere-D5Mit254-18.0 +_ grt, D5Mit240, D5Mit314, D5M#338 3.1 cM-grt, D5Mit240, D5Mit314, D5Mit338-15.3 +_ 2.9 cM- D5Mit29-telomere Method of mapping: (C57BL/6 x DW/J-grt)F 1 � DW/J-grt back- 15 ":1 DSMit29 cross mice. Haplotype analysis is shown in Fig. 1A. Database deposit information: MGD accession number, MGD- JNUM-40511 Molecular reagents: Microsatellite primers for PCR used in this study were all purchased from Research Genetics, Inc. (Huntsville, Fig, 1. Chromosomal localization of the grt in the mouse genome. (A) Ala., USA). Haplotype analysis of 150 (C57BL/6 x DW/J-grt)F~ x DW/J-grt back- Allele detection: Genotype of the grt/grt or grt/+ was determined crosses. Loci followed are listed on the left. Each column represents the by weighing mice at 6 weeks of age. chromosome identified in backcrosses inherited from the (C57BL/6 x DW/ Linkage analysis was performed in 150 backcross progeny by J-grt)F 1 parent. Black squares represent the C57BL/6 allele; white squares the grt genotype with DW/J-grt allele for several microsatellite represent the DW/J-grt allele. The number of backcrosses carrying each loci representative of each chromosome. Since some of microsat- haplotype is listed at the bottom. (B) Genetic linkage map showing the location of the grt. The loci typed in the backcross are on the right, with ellite loci localized on Chr 5 showed a cross-linkage to the grt distances between the loci given in centimorgan. allele, other microsatellite loci on Chr 5 were further examined. Finally, three microsatellite alleles were found to show no recom- bination with the grt allele (Fig. 1). Thus, the grt locus was 3. Sornson MW, Wu W, Dasen JS, Flynn SE, Norman DJ, O'Connell SM, Gukovsky I, Carriere C, Ryan AK, Miller AP, Zuo L, Gleiberman AS, mapped on 59 cM distal from the centromere of Chr 5, where Andersen B, Beamer WG, Rosenfeld MG (1996) Nature 384, 327-333 human Chr 22ql 1.2-q13.1 shows synteny. 4. Lin S-C, Lin CR, Gukovsky I, Lusis AJ, Sawchenko PE, Rosenfeld MG Discussion: Several mouse mutants exhibiting dwarfism have (1993) Nature 364, 209-213 been reported. The responsible genes for them have already been 5. Godfrey P, Rahal JO, Beamer WG, Copeland NG, Jenkins NA, Mayo mapped and cloned [1-6]; dw (Chr 16), df (Chr 11), lit (Chr 6), and KE (1993) Nature Genet 4, 227-232 hyt (Chr 12). Mutant mice exhibiting dwarfism occurred recently 6. Stein SA, Oates EL, Hall CR, Glumbles RM, Fernandez LM, Taylor in our DW/J-+/dw colony. Their phenotype was not as the dw NA, Puett D, Jin S (1994) Mol Endocrinol 8, 129-138 phenotype in physiological and histological studies [7,8]; dwarf- 7. Yoshida T, Yamanaka K, Atsumi S, Tsumura H, Sasaki R, Tomita K, ism in this mouse was thought to be caused by an abnormality in Ishikawa E, Ozawa H, Watanabe K, Totsuka T (1994) J Endocrinol 142, 435-446 the thyroid function, whereas the primary cause in dw mice was 8. Tomita K, Yoshida T, Morita J, Atsumi S, Totsuka T (1995) J Endo- functional deficiency in the transcription factor, Pit-l, specifically crinol 144, 209-214 localized in the pituitary. Thus, we termed this putative locus growth-retarded (grt). In this report, the grt locus was shown to be localized on Chr 5, verifying that the grt gene is different from the Mapping of the mouse corticotropin-releasing dw or other murine mutant genes causing dwarfism reported so far. hormone receptor 2 gene (Crhr2) to References Chromosome 6 1. Camper SA, Saunders TL, Katz RW, Reeves RH (1990) Genomics 8, 586-590 J. Shonee Lesh, 1 Heather L. Burrows, 2"~ 2. Li S, Crenshaw EB, Rawson EJ, Simmons DM, Swanson LW, Rosen- feld MG (1990) Nature 347, 528-533. Audrey F. Seasholtz, 1'4 Sally A. Camper 2 Correspondence to: T. Agui Correspondence to: S.A. Camper Mammalian Genome Springer Journals

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