Brief Data Reports Mammalian Genome 8,376-384 (1997). 9 Springer-Verlag New York Inc. 1997 Mapping of the eukaryotic initiation factor elF-1A gene, Eifla, to mouse Chromosome 12D-E by FISH N. Kikyo, M. Tada, T. Tada, M.A. Surani Wellcome/CRC Institute of Cancer and Developmental Biology, and Physiological Laboratory, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK Received: 31 October 1996 / Accepted: 12 January 1997 Species: Mouse Locus name: Eukaryotic initiation factor 1A (eIF-1A) Locus symbol: Eifla Map position: mouse Chromosome (Chr) 12 band D-E Method of mapping: FISH. The cDNA probe was labeled with biotin-16-dUTP, and the signal was detected by streptavidin-FITC (BRL) and biotinylated anti-avidin D (Vector) as described . Chromosomes were counterstained with propidium iodide and G- banded with DAPI. Database deposit information: GenBank accession number for sequence is U28419. Mouse Genome Database accession number for mapping is MGD-INEX-33. Molecular reagents used for mapping: D52, a 1.9-kb cDNA probe encoding Eifla was used in FISH. Previously identified homologs: Saccharomyces cerevisiae homo- log, TIFll , is at YMR260C. Human, rabbit, and wheat ho- mologs  have not been mapped yet. Fig. 1. FISH analysis of the Eifla gene. (A) Arrows indicate signals on Chr 12. (B) G-banding of the mitotic chromosome spread shown in A. Discussion: In FISH analysis for the Eifla gene, doublet signals were located on mouse chromosome band 12D in ten mitotic spreads (Fig. 1) and on 12E in three spreads. In addition, in six ported by the Sankyo Foundation of Life Science, the Nakayama Founda- spreads we could not discriminate between 12D or 12E. We there- tion for Human Science, and the Daiwa Anglo-Japanese Foundation. M. fore conclude that Eifla maps close to the D-E boundary of Chr Tada was supported by the Japan Society for the Promotion of Science. 12. It is unlikely that we were detecting two different loci on Chr References 12 since genomic Southern analysis with the same cDNA probe 1. Pinkel D, Straume T, Gray JW (1986) Proc Natl Acad Sci USA 83, detected a single band in DNA digested with a range of restriction 2934-2938 enzymes (data not shown). Experiments using a mouse-hamster 2. Wei C-L, Kainuma M, Hershey JWB (1995) J Biol Chem 270, 22788- somatic cell hybrid panel also indicated Eifla maps to mouse Chr 12 (data not shown). 3. Dever TE, Wei C-L, Benkowski LA, Browning K, Merrick WC, Her- Eukaryotic initiation factor 1A (formerly called eIF-4C) func- shey JWB (1994) J Biol Chem 269, 3212-3218 tions in the early steps of translation by promoting the dissociation 4. Hershey JWB (1991) Annu Rev Biochem 60, 717-755 of 80S ribosomes into 40S and 60S subunits and stabilizing a 5. Davis W. Jr, De Sousa PA, Schultz RM (1996) Dev Biol 174, 190-201 preinitiation complex composed of initiator methionyl-tRNA (Met-tRNA,), eIF-2, GTP, and 40S ribosomal subunit . Mouse eDNA for Eifla has been cloned recently, and its expression pat- The mouse synaptonemal complex protein tern in the early development was analyzed . The transcription gene Sycp3 maps to band C of of Eifla transiently increases at the two-cell stage, for which the Chromosome 10 first round of DNA replication is critical. The following decrease of transcription is mediated by histone deacetylation, which was shown by using an inhibitor of histone deacetylase, trapoxin. Thus, Albrecht Klink, Muriel Lee, Howard J. Cooke the study of transcriptional regulation of Eifla provides insight into the mechanisms of zygotic gene activation in terms of chro- MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK matin structure. Acknowledgments: We thank H. Niwa for providing the eDNA library Received: 9 November 1996 / Accepted: 28 December 1996 from ES cells and W. M. Rideout III for technical assistance. This work Species: Mouse (Mus musculus) was supported by the Wellcome Trust (grant No. 036481) and the Human Frontier Science Program (grant No. RG-516/94M). N. Kikyo was sup- Locus name: Synaptonemal complex protein 3 Correspondence to: M.A. Surani Correspondence to: H.J. Cooke
Mammalian Genome – Springer Journals
Published: Mar 21, 2009
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