Mammary stem cell and macrophage markers are enriched in normal tissue adjacent to inflammatory breast cancer

Mammary stem cell and macrophage markers are enriched in normal tissue adjacent to inflammatory... Introduction We hypothesized that breast tissue not involved by tumor in inflammatory breast cancer (IBC) patients con- tains intrinsic differences, including increased mammary stem cells and macrophage infiltration, which may promote the IBC phenotype. Materials and methods Normal breast parenchyma ≥ 5 cm away from primary tumors was obtained from mastectomy specimens. This included an initial cohort of 8 IBC patients and 60 non-IBC patients followed by a validation cohort of 19 + + + IBC patients and 25 non-IBC patients. Samples were immunostained for either CD44 CD49f CD133/2 mammary stem cell markers or the CD68 macrophage marker and correlated with IBC status. Quantitation of positive cells was determined using inForm software from PerkinElmer. We also examined the association between IBC status and previously published tumorigenic stem cell and IBC tumor signatures in the validation cohort samples. + + + Results 8 of 8 IBC samples expressed isolated CD44 CD49f CD133/2 stem cell marked cells in the initial cohort as + + + opposed to 0/60 non-IBC samples (p = 0.001). Similarly, the median number of CD44 CD49f CD133/2 cells was signifi- cantly higher in the IBC validation cohort as opposed to the non-IBC validation cohort (25.7 vs. 14.2, p http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Breast Cancer Research and Treatment Springer Journals
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Publisher
Springer US
Copyright
Copyright © 2018 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Medicine & Public Health; Oncology
ISSN
0167-6806
eISSN
1573-7217
D.O.I.
10.1007/s10549-018-4835-6
Publisher site
See Article on Publisher Site

Abstract

Introduction We hypothesized that breast tissue not involved by tumor in inflammatory breast cancer (IBC) patients con- tains intrinsic differences, including increased mammary stem cells and macrophage infiltration, which may promote the IBC phenotype. Materials and methods Normal breast parenchyma ≥ 5 cm away from primary tumors was obtained from mastectomy specimens. This included an initial cohort of 8 IBC patients and 60 non-IBC patients followed by a validation cohort of 19 + + + IBC patients and 25 non-IBC patients. Samples were immunostained for either CD44 CD49f CD133/2 mammary stem cell markers or the CD68 macrophage marker and correlated with IBC status. Quantitation of positive cells was determined using inForm software from PerkinElmer. We also examined the association between IBC status and previously published tumorigenic stem cell and IBC tumor signatures in the validation cohort samples. + + + Results 8 of 8 IBC samples expressed isolated CD44 CD49f CD133/2 stem cell marked cells in the initial cohort as + + + opposed to 0/60 non-IBC samples (p = 0.001). Similarly, the median number of CD44 CD49f CD133/2 cells was signifi- cantly higher in the IBC validation cohort as opposed to the non-IBC validation cohort (25.7 vs. 14.2, p

Journal

Breast Cancer Research and TreatmentSpringer Journals

Published: Jun 1, 2018

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