A decrease in ambient temperature induced the desC gene encoding the only acyl-lipid desaturase of thermophilic cyanobacterium Synechococcus vulcanus. The addition of the substrate for this enzyme, stearic acid (18:0), to the culture medium of cyanobacterium grown at optimum temperature also induced the desC gene. At 55 and 45°C, gene transcription started at the position –59 upstream of the translation initiation codon. After addition of 18:0, two specific pools of transcripts were detected. Some transcripts corresponded in size to transcripts synthesized at 55 and 45°C, and other transcripts were longer. The determination of sites of the transcript synthesis initiation showed that 18:0 additionally induced transcription from positions –163 and –169. These additional initiation sites were positioned within the coding sequence of the hemN gene preceding the desC gene. Thus, at low-temperature and substrate induction, the expression of the desC gene was evidently controlled by different promoter sequences.
Russian Journal of Plant Physiology – Springer Journals
Published: Oct 18, 2004
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