Low-Temperature and Substrate Induction of the Gene for Δ9 Fatty Acid Desaturase in the Thermophilic Cyanobacterium Synechococcus vulcanus

Low-Temperature and Substrate Induction of the Gene for Δ9 Fatty Acid Desaturase in the... A decrease in ambient temperature induced the desC gene encoding the only acyl-lipid desaturase of thermophilic cyanobacterium Synechococcus vulcanus. The addition of the substrate for this enzyme, stearic acid (18:0), to the culture medium of cyanobacterium grown at optimum temperature also induced the desC gene. At 55 and 45°C, gene transcription started at the position –59 upstream of the translation initiation codon. After addition of 18:0, two specific pools of transcripts were detected. Some transcripts corresponded in size to transcripts synthesized at 55 and 45°C, and other transcripts were longer. The determination of sites of the transcript synthesis initiation showed that 18:0 additionally induced transcription from positions –163 and –169. These additional initiation sites were positioned within the coding sequence of the hemN gene preceding the desC gene. Thus, at low-temperature and substrate induction, the expression of the desC gene was evidently controlled by different promoter sequences. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Low-Temperature and Substrate Induction of the Gene for Δ9 Fatty Acid Desaturase in the Thermophilic Cyanobacterium Synechococcus vulcanus

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Publisher
Kluwer Academic Publishers-Plenum Publishers
Copyright
Copyright © 2004 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1023/B:RUPP.0000019208.08374.54
Publisher site
See Article on Publisher Site

Abstract

A decrease in ambient temperature induced the desC gene encoding the only acyl-lipid desaturase of thermophilic cyanobacterium Synechococcus vulcanus. The addition of the substrate for this enzyme, stearic acid (18:0), to the culture medium of cyanobacterium grown at optimum temperature also induced the desC gene. At 55 and 45°C, gene transcription started at the position –59 upstream of the translation initiation codon. After addition of 18:0, two specific pools of transcripts were detected. Some transcripts corresponded in size to transcripts synthesized at 55 and 45°C, and other transcripts were longer. The determination of sites of the transcript synthesis initiation showed that 18:0 additionally induced transcription from positions –163 and –169. These additional initiation sites were positioned within the coding sequence of the hemN gene preceding the desC gene. Thus, at low-temperature and substrate induction, the expression of the desC gene was evidently controlled by different promoter sequences.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Oct 18, 2004

References

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