Long-term T-DNA insert stability and transgene expression consistency in field propagated sugarcane

Long-term T-DNA insert stability and transgene expression consistency in field propagated sugarcane Plant Mol Biol (2017) 93:451–463 DOI 10.1007/s11103-016-0572-6 Long-term T-DNA insert stability and transgene expression consistency in field propagated sugarcane 1 1 2 1 1 1 Kerry Hosmer Caffall  · Chengkun He  · Michele Smith-Jones  · Kristin Mayo  · Pearl Mai  · Shujie Dong  · 1 1 1 1 1 1 4 John Ke  · Erik Dunder  · Michele Yarnall  · Rachel Whinna  · Joe DeMaio  · Weining Gu  · Judith Sheldon  · 1 1 1 3 3 3 3 Martin Allen  · Tricia Costello  · Kristin Setliff  · Rakesh Jain  · Ada Snyder  · Clark Lovelady  · Eric Rawls  · 3 1 1 1 1 Eric Palmer  · Yan Zhang  · Nicholas Bate  · Liang Shi  · Ian Jepson   Received: 12 January 2016 / Accepted: 2 December 2016 / Published online: 28 December 2016 © Springer Science+Business Media Dordrecht 2016 Abstract of field propagated sugarcane. These data are critical sup- Key message This study addresses T-DNA insert sta- porting information needed for successful commercializa- bility and transgene expression consistency in multiple tion of GM sugarcane. Here seventeen transgenic events, cycles of field propagated sugarcane. T-DNA inserts are containing the AmCYAN1 gene driven by a CMP promoter stable; no transgene rearrangements were observed. and the E. coli PMI gene driven by either a CMP or Ubi AmCYAN1 and PMI protein accumulation levels were promoter, were used to http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

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Springer Netherlands
Copyright © 2016 by Springer Science+Business Media Dordrecht
Life Sciences; Plant Sciences; Biochemistry, general; Plant Pathology
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