Location and architecture of an antibody-binding site of influenza A virus nucleoprotein

Location and architecture of an antibody-binding site of influenza A virus nucleoprotein Amino acid positions recognized by monoclonal antibodies (MAbs) in the influenza A virus nucleoprotein (NP) have been reported. As these residues were scattered in the three-dimensional (3D) structure of NP, no patterns of the architecture of antibody-binding sites could be inferred. Here, we used site-specific mutagenesis and ELISA to screen the amino acids surrounding position 470 recognized by the MAb 3/1 as a linear epitope. Ten amino acid residues involved in the reaction of NP with the MAb 3/1 and the MAb 469/6 were identified. Our data are the first to outline a compact site recognized by MAbs in the 3D structure of the influenza virus NP. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Location and architecture of an antibody-binding site of influenza A virus nucleoprotein

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Publisher
Springer Journals
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1952-8
Publisher site
See Article on Publisher Site

Abstract

Amino acid positions recognized by monoclonal antibodies (MAbs) in the influenza A virus nucleoprotein (NP) have been reported. As these residues were scattered in the three-dimensional (3D) structure of NP, no patterns of the architecture of antibody-binding sites could be inferred. Here, we used site-specific mutagenesis and ELISA to screen the amino acids surrounding position 470 recognized by the MAb 3/1 as a linear epitope. Ten amino acid residues involved in the reaction of NP with the MAb 3/1 and the MAb 469/6 were identified. Our data are the first to outline a compact site recognized by MAbs in the 3D structure of the influenza virus NP.

Journal

Archives of VirologySpringer Journals

Published: Jun 1, 2014

References

  • Protection against multiple influenza A virus subtypes by intranasal administration of recombinant nucleoprotein
    Guo, L; Zheng, M; Ding, Y; Li, D; Yang, Z; Wang, H; Chen, Q; Sui, Z; Fang, F; Chen, Z
  • Antigenically relevant amino acid positions as revealed in reactions of monoclonal antibodies with the nucleoproteins of closely related influenza A virus strains
    Varich, NL; Kaverin, NV
  • Development and application of monoclonal antibodies against avian influenza virus nucleoprotein
    Yang, M; Berhane, Y; Salo, T; Li, M; Hole, K; Clavijo, A
  • Monoclonal antibodies specific for discontinuous epitopes direct refolding of influenza A virus hemagglutinin
    Yewdell, JW

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