Mammalian Genome 9, 476–478 (1998). Incorporating Mouse Genome © Springer-Verlag New York Inc. 1998 Localization of the rat M1 muscarinic receptor gene to Chromosome 1q43-51 1 2 2 3 Christoph P.R. Klett, Drazen B. Zimonjic, Catherine L. Keck, Tom I. Bonner Department of Pharmacology, University of California at San Diego, La Jolla, California 92093-0636, USA Laboratory of Experimental Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20892, USA National Institute of Mental Health, Section on Genetics, Bethesda, Maryland 20892, USA Received: 4 December 1997 / Accepted: 11 February 1998 Muscarinic acetyl choline receptors are widely distributed in the SSCP analysis, 5 ml of the PCR product was denatured in the brain (Levy et al. 1991). Five different subtypes have been iden- presence of 80 mM methylmercury hydroxide, then heated to 85°C tified that play important roles in pyramidal function, parasympa- for 4 min and immediately quenched on ice before being loaded thetic regulation, and in the learning process (Wei et al. 1994). onto the gel. Under these conditions, SSCP analysis of the DNA Central muscarinic receptors, and specifically the M1 muscarinic fragments derived from the rat/mouse somatic cell hybrid panel receptor, have further been hypothesized as contributory to differ- DNAs revealed
Mammalian Genome – Springer Journals
Published: Jun 1, 1998
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