Localization of the mitochondrial carbonic anhydrase V gene, Car5, on mouse chromosome 8

Localization of the mitochondrial carbonic anhydrase V gene, Car5, on mouse chromosome 8 Mammalian Genome 8, Brief Data Reports 225 Database deposit information: Complete public raw data from The Jackson Laboratory BSB and BSS crosses with references and notes can be obtained from the World Wide Web address, http:// www.jax.org/resources/documents/cmdata. MGD accession num- bers: BSB cross = MGD-CREX-702; BSS cross = MGD-CREX- Molecular reagents: Two primers for the polymerase chain reac- tion (PCR) were designed based on the mouse Car5 sequence [3] to amplify the 3'-UTR. The forward primer was 5'-AGGATGAA- GTCTGTAAGGATAGGCAGAGCA-3' and the reverse primer was 5'-TTCTCTGTGTAGTCCTGGCTGTCCTGGAA-3'. Allele detection: A strain difference was detected by PCR. The PCR conditions consisted of a predenaturation step at 94~ for 2 min, followed by 30 cycles at 94~ for 45 s, 59~ for 1 min, and 72~ for 1 rain. The PCR products were about 200 bp and 250 bp in the C57BL/6J and M. spretus DNAs, respectively. Previously identified homologs: rat CA V [4]; human CA V[5]. Discussion: The mouse Car5 gene encodes a 298-amino acid pre- cursor prepropeptide whose 28-amino acid leader sequence is pro- teolytically cleaved to produce a mature 270 amino acid CA V protein localized to the mitochondrial matrix [4]. In mouse, CA V appears to be expressed primarily http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mammalian Genome Springer Journals

Localization of the mitochondrial carbonic anhydrase V gene, Car5, on mouse chromosome 8

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Copyright © 1997 by Springer-Verlag
Life Sciences; Cell Biology; Anatomy; Zoology
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