Mammalian Genome 8, Brief Data Reports 225 Database deposit information: Complete public raw data from The Jackson Laboratory BSB and BSS crosses with references and notes can be obtained from the World Wide Web address, http:// www.jax.org/resources/documents/cmdata. MGD accession num- bers: BSB cross = MGD-CREX-702; BSS cross = MGD-CREX- Molecular reagents: Two primers for the polymerase chain reac- tion (PCR) were designed based on the mouse Car5 sequence  to amplify the 3'-UTR. The forward primer was 5'-AGGATGAA- GTCTGTAAGGATAGGCAGAGCA-3' and the reverse primer was 5'-TTCTCTGTGTAGTCCTGGCTGTCCTGGAA-3'. Allele detection: A strain difference was detected by PCR. The PCR conditions consisted of a predenaturation step at 94~ for 2 min, followed by 30 cycles at 94~ for 45 s, 59~ for 1 min, and 72~ for 1 rain. The PCR products were about 200 bp and 250 bp in the C57BL/6J and M. spretus DNAs, respectively. Previously identified homologs: rat CA V ; human CA V. Discussion: The mouse Car5 gene encodes a 298-amino acid pre- cursor prepropeptide whose 28-amino acid leader sequence is pro- teolytically cleaved to produce a mature 270 amino acid CA V protein localized to the mitochondrial matrix . In mouse, CA V appears to be expressed primarily
Mammalian Genome – Springer Journals
Published: Mar 23, 2009
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera