Localization of the gene encoding a novel isoform of lysyl hydroxylase

Localization of the gene encoding a novel isoform of lysyl hydroxylase Mammalian Genome 8, Brief Data Reports 707 Method of mapping: FISH and in situ hybridization Localization of the gene encoding a novel Database depository information: GenBank J02921, M35368, isoform of lysyl hydroxylase M36682, M64303 Molecular reagents: FISH localization: a genomic probe contain- Claude Szpirer, 1 Josiane Szpirer, 1 Mich~le Rivi~re, 1 ing 12 kb of human DNA spanning the complete human galectin-3 gene was labeled with biotin or digoxigenin (Random Primed Pascale Vanvooren, ~ Minna Valtavaara, 2 Raili Myllyl~i 2 DNA labeling Kit, Boehringer-Mannheim, Germany). The labeled ~Drpartement de Biologie Molrculaire, Universit6 Libre de Bruxelles, probe was used for in situ hybridization of human chromosomes Rue des Chevaux, 67, B-1640 Rhode-St-Genbse, Belgium derived from methotrexate-synchronized normal peripheral lym- ZDepartment of Biochemistry, University of Oulu, Linnanmaa, phocyte cultures. The conditions of hybridization, the detection of FIN-90570 Oulu, Finland hybridization signals, digital-image acquisition, processing, and analysis as well as the procedure for direct visualization of fluo- Received: 27 January 1997/Accepted: 11 April 1997 rescent signals to banded chromosomes were performed as previ- ously described [1,2]. Isotypic in situ hybridization was performed Species: Human and rat in the conditions described previously [3] with a tritium-labeled Locus name: Procollagen-lysine, 2-oxoglutarate 5-dioxygenase or probe http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mammalian Genome Springer Journals

Localization of the gene encoding a novel isoform of lysyl hydroxylase

Loading next page...
 
/lp/springer_journal/localization-of-the-gene-encoding-a-novel-isoform-of-lysyl-hydroxylase-ywuStQxaKV
Publisher
Springer-Verlag
Copyright
Copyright © 1997 by Springer-Verlag
Subject
Life Sciences; Cell Biology; Anatomy; Zoology
ISSN
0938-8990
eISSN
1432-1777
D.O.I.
10.1007/s003359900549
Publisher site
See Article on Publisher Site

Abstract

Mammalian Genome 8, Brief Data Reports 707 Method of mapping: FISH and in situ hybridization Localization of the gene encoding a novel Database depository information: GenBank J02921, M35368, isoform of lysyl hydroxylase M36682, M64303 Molecular reagents: FISH localization: a genomic probe contain- Claude Szpirer, 1 Josiane Szpirer, 1 Mich~le Rivi~re, 1 ing 12 kb of human DNA spanning the complete human galectin-3 gene was labeled with biotin or digoxigenin (Random Primed Pascale Vanvooren, ~ Minna Valtavaara, 2 Raili Myllyl~i 2 DNA labeling Kit, Boehringer-Mannheim, Germany). The labeled ~Drpartement de Biologie Molrculaire, Universit6 Libre de Bruxelles, probe was used for in situ hybridization of human chromosomes Rue des Chevaux, 67, B-1640 Rhode-St-Genbse, Belgium derived from methotrexate-synchronized normal peripheral lym- ZDepartment of Biochemistry, University of Oulu, Linnanmaa, phocyte cultures. The conditions of hybridization, the detection of FIN-90570 Oulu, Finland hybridization signals, digital-image acquisition, processing, and analysis as well as the procedure for direct visualization of fluo- Received: 27 January 1997/Accepted: 11 April 1997 rescent signals to banded chromosomes were performed as previ- ously described [1,2]. Isotypic in situ hybridization was performed Species: Human and rat in the conditions described previously [3] with a tritium-labeled Locus name: Procollagen-lysine, 2-oxoglutarate 5-dioxygenase or probe

Journal

Mammalian GenomeSpringer Journals

Published: Mar 31, 2009

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off