Mammalian Genome 8, Brief Data Reports 707 Method of mapping: FISH and in situ hybridization Localization of the gene encoding a novel Database depository information: GenBank J02921, M35368, isoform of lysyl hydroxylase M36682, M64303 Molecular reagents: FISH localization: a genomic probe contain- Claude Szpirer, 1 Josiane Szpirer, 1 Mich~le Rivi~re, 1 ing 12 kb of human DNA spanning the complete human galectin-3 gene was labeled with biotin or digoxigenin (Random Primed Pascale Vanvooren, ~ Minna Valtavaara, 2 Raili Myllyl~i 2 DNA labeling Kit, Boehringer-Mannheim, Germany). The labeled ~Drpartement de Biologie Molrculaire, Universit6 Libre de Bruxelles, probe was used for in situ hybridization of human chromosomes Rue des Chevaux, 67, B-1640 Rhode-St-Genbse, Belgium derived from methotrexate-synchronized normal peripheral lym- ZDepartment of Biochemistry, University of Oulu, Linnanmaa, phocyte cultures. The conditions of hybridization, the detection of FIN-90570 Oulu, Finland hybridization signals, digital-image acquisition, processing, and analysis as well as the procedure for direct visualization of fluo- Received: 27 January 1997/Accepted: 11 April 1997 rescent signals to banded chromosomes were performed as previ- ously described [1,2]. Isotypic in situ hybridization was performed Species: Human and rat in the conditions described previously  with a tritium-labeled Locus name: Procollagen-lysine, 2-oxoglutarate 5-dioxygenase or probe
Mammalian Genome – Springer Journals
Published: Mar 31, 2009
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