948 Mammalian Genome 8, Brief Data Reports The organization of the pig B2M gene is not known, although its Localization of the beta2-microglobulin gene corresponding cDNA sequence has been established . The de- to pig Chromosome lq17 rived amino acid sequence revealed 72% identity with its human counterpart. At the molecular level, the alignment of the pig B2M C. Rogel-Gaillard, l M. Vaiman, 1 C. Renard, 1 cDNA (Acc. No. L13854) with the human B2M gene (Acc. No. P. Chardon, 1 M. Yerle z M17986)  allowed the identification of the different exons. A PCR primer pair was derived from the pig sequence corresponding 1Laboratoire mixte INRA-CEA de Radiobiologie appliquee, DSV, DRR, to the exon 2 in human. The resulting PCR product on pig genomic LRA, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France DNA was obtained at an annealing temperature of 60~ with the 2Laboratoire de GrnEtique cellulaire, INRA BP27, F-31326 Amplitaq enzyme (Perkin-Elmer). The amplified fragment was Castanet Tolosan, France checked by sequencing and was identical to the previously pub- lished sequence . PCR screening of the somatic cell hybrids Received: 30 May 1997 / Accepted: 23 July 1997 revealed that the pig B2M gene mapped to Chr I in the region Species: Pig (Sus scrofa domestica) ql 1--<t17 with a probability of 81% . The FISH mapping un- Locus name: Beta2-microglobulin ambiguously localized the B2M gene on SSClqI7 for both YACs, Locus symbol: B2M in spite of extra signals on Chr 13 for YAC 163A06 (Fig. 1). Note Map position: lq17 that this localization is in agreement with the human-pig compara- Method of mapping: PCR screening on a panel of 27 pig-rodent tive cytogenetic map  and adds a new marker to the conserved somatic cell hybrids  and fluorescence in situ hybridization on CYP19-IGF1R synthenic group between HSA15 and SSC1. In pig metaphases. conclusion, as in humans and mice, the pig B2M gene does not Molecular reagents: Primers designed from the pig B2M cDNA belong to the Mhc region that is assigned near the centromere of . The direct primer was 5'GGTTCAGGTTTACTCACGC- Chr 7 in pig. CAC 3' (position 135-156), and the reverse primer was 5'CT- TAACTATCTTGGGCTTATCG 3" (position 396--375). The gen- erated PCR product was 262 bp in size. The FISH experiment was References performed with 2 YACs recovered by PCR screening from our pig library , including a 1-Mb YAC (163A06) and a 120-kb YAC 1. Yerle M, Echard G, Robic A, Mairal A, Dubut-Fontana C, Riquet J, (171A09). Pinton P, Milan D, Lahbib-Mansais Y, Gellin J (1996) Cytogenet Cell Previously identified homologs: The corresponding human B2M Genet 73, 194-202 gene has been mapped on Chromosome (Cbr) 15q21-q22.2 . 2. Milland J, Loveland BE, McKenzie IFC (1993) Immunogenetics 38, Results and Discussion: The ~2 M protein, which is a member of the immunoglobulin superfamily, forms a non-covalent associa- 3. Rogel-Gaillard C, Bourgeaux N, Save JC, Renard C, Coullin P, Pinton tion with the extracellular domain of the major histocompatibility P, Yerle M, Valman M, Chardon P (1997) Mamm Genome 8, 186-192 complex (Mhc) classical class I  as well as class I-related mol- 4. Pajunen L, Solomon E, i3urgess S, Bobrow M, Povey S, Swallow D ecules such as the human CD1 antigens . This 11.7-kDa protein (1978) Cytogenet Cell Genet 22, 511-512 also forms heterodimers with the more distantly class I-related Fc 5. Gtissow D, Rein R, Ginjaar I, Hochstenbach F, Seeman G, Kottman A, receptor that is expressed in the intestinal epithelial cells of neo- Ploegh HL (1987) J Immunol 139, 3132-3138 natal rats (FcRn) or mice . In addition, the 132M protein is present as a free form in various physiological fluids. The role of 6. Calabi F, Bradbnry A (1991) Tissue Antigens 37, 1-9 the [~2M protein is crucial to obtain correct class I molecule three- 7. Simister NE, Mostov KE (1989) Nature 337, 184-187 dimensional folding, allowing adequate groove loading of endog- 8. Chardon P, Vaiman M, Renard C, Arnoux B (1978) Transplantation enously processed peptides, normal intracellular trafficking of the 26, 107-112 complex, and finally stable expression at the cell surface . In 9. Gtissow D, Rein R, Ginjaar I, Hochstenbach F, Seeman G, Kottman A, pig, the [3aM protein was also shown to be non-covalently asso- Ploegh HL (1987) J Immunol 139, 3132-3138 ciated with the various expressed Mhc class I polymorphic heavy 10. Chevalet C, Gouzy I, San Cristobal-Gaudy M (1997) Comput Appl chains . Biosci 13, 69-73 11. Goureau A, Yerle M, Schmitz A, Riquet J, Milan D, Pinton P, Frelat Correspondence to: C. Rogel-Galllard G, Gellin J (1996) Genomics 36, 252-262 Fig. 1. Regional localization of B2M gene by FISH. (a) YAC 163A06: a bright hybridization signal is observed on pig Chr 1 in the q17 region, and a secondary signal is detected on Chr 13q33, indicating that this YAC is chimeric. (b) YAC 171A09: a strong hybridization signal is observed on pig Chr 1 in the q17 region.
Mammalian Genome – Springer Journals
Published: Mar 21, 2009
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