Mammalian Genome 8, Brief Data Reports 73 Physical and genetic mapping of the prosaposin gene (PSAP) to bovine Chromosome 28 C. Elduque, 1 L. Li, 2 W. Barendse, 3 J.E. Womack 2 1Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain 2Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas 77843, USA 3CSIRO, Division of Tropical Animal Production, Research Road, University of Queensland, St. Lucia, Brisbane, 4072, Australia Received: 3 July 1996 / Accepted: 27 August 1996 Species: Cattle Locus name: Prosaposin Fig. 1. Segregation in two cattle Locus symbol: PSAP families of PSAP polymorphisms Map position: BTA28. Linkage was detected to the anonymous after digestion with HindlIl (A) and DNA polymorphism D28S4 (theta 0.22, Iodscore 4.50). PstI (B) and probed with a human Method of mapping: Southern analysis of somatic cell hybrids  PSAP probe. and the International Bovine Reference Family Panel (IBRP) . Molecular reagents: human SAP-2 eDNA probe SAP4B (ATCC# Further studies of the linkage order among the Type I loci 59662). Filters were hybridized at 42~ in 50% formamide and assigned to BTA28 will reveal whether the syntenic conservation washed at low stringency (1 x SSC, 0.1% SDS at 55~ of
Mammalian Genome – Springer Journals
Published: Mar 23, 2009
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