Earlier, polyclonal antibodies to the Escherichia coliRecA protein revealed immunologically related proteins only in the reproductive tissues of various eukaryotes and the proteins were localized to the various structures of the synaptonemal complex (SC). Indirect immunofluorescent FITC staining with polyclonal antibodies against RecA detected RecA-like antigens in the cell center (centrosome) and the replicated centrioles of fixed HeLa and K-562 cells. Human and mouse genes, which were previously cloned by immunological relatedness of their products to E. coliRecA, were used as hybridization probes in Northern blot analysis of the transcription activity of similar genes in K-562 cells. A gene homologous to the cloned mouse gene proved transcribed in the cells studied.
Russian Journal of Genetics – Springer Journals
Published: Oct 8, 2004
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