Cancer Gene Therapy (2018) 25:39–46
LncRNA SPRY4-IT was concerned with the poor prognosis and
contributed to the progression of thyroid cancer
Received: 28 June 2017 / Revised: 9 August 2017 / Accepted: 16 August 2017 / Published online: 12 December 2017
© Nature America, Inc., part of Springer Nature 2018
Long non-coding RNAs (lncRNAs) have been demonstrated to be critical regulators in tumorigenesis. LncRNA SPRY4-IT1
has been identiﬁed as critical regulator for hepatocellular carcinoma and ovarian cancer. However, the potential role and
clinical value of SPRY4-IT1 in human thyroid cancer (TC) still remain unclear and need to be uncovered. Our current study
was aimed to ascertain the biological role of expression of SPRY4-IT1 in TC tissues and cells. Our ﬁndings revealed that the
level of SPRY4-IT1 was signiﬁcantly upregulated in TC tissues and cell lines, which was correlated with poor prognosis.
And cellular experiments exhibited that silenced SPRY4-IT1 inhibited the proliferative and migratory abilities of TC cells.
Mechanism assays noted that silenced SPRY4-IT1 could increase the levels of transforming growth factor-β1 (TGF-β1) and
p-Smad2/3 and function mediated by si-SPRY4-IT1 could be rescued by the interference of TGF-β1. Generally speaking,
these ﬁndings elucidated that SPRY4-IT1 might become a novel prognostic factor in the clinical behaviors of TC patients
and participated in the progression of TC through targeting TGF-β/Smad signaling pathway.
Thyroid cancer (TC) is one of the malignant tumors in
endocrine organs whose incidence rate has been increasing
over the past 10 years . Despite many improvements in
treatment have been explored, the rate of 5-year survival for
TC is still unsatisﬁed [2, 3]. TC is identiﬁed as a genic
disease with genetic and epigenetic alterations, therefore,
investigating the mechanism behind the initiation and
progression of TC is crucial for exploring novel treatment
Long non-coding RNAs (lncRNAs) have a length >200
nucleotides and are not capable of coding proteins .
Currently, it has been documented that the dysregulation of
lncRNAs are involved in various biological functions. For
example, previous studies proved that lncRNA MALAT1
could facilitate the progression of HCC via improving
the level of SRSF1 and activating mammalian target
of rapamycin . According to Gupta et al., lncRNA
HOTAIR could promote metastasis in tumors through
making chromatin to be reprogramed . Additionally,
there are many novel lncRNAs such as UFC1, ZFAS1,
CDRIAS, T-UCR, and CASC2, which also have been
reported to participate in the tumorigenesis [7–10]. Despite
so many lncRNAs that have been reported recently, little
has been investigated in the relationship between lncRNAs
and TC progression.
SPRY4-intronic transcript 1 (SPRY4-IT1, NC_000005)
has been identiﬁed as an oncogene in various cancers.
Overexpression of SPRY4-IT1 has been identiﬁed in hepa-
tocellular carcinoma, gallbladder carcinoma, osteosarcoma
cell, cervical cancer, and colorectal cancer, and high level of
SPRY4-IT1 has strong correlation with the undesirable
prognosis [11–18]. However, expression pattern, clinical
value, biological roles, and connotative mechanism of
SPRY4-IT1 in TC have not been investigated.
Haoyu Zhou and Zhihua Sun contributed equally to this work.
* Xiaofeng Wang
* Xuejun Zhou
School of Food Science, Henan Institute of Science and
Technology, Xinxiang, Henan Province 453000, China
Institute of Tropical Agriculture and Forestry, Hainan University,
Haikou, Hainan Province 570228, China
School of resources and environment, Henan Institute of Science
and Technology, Xinxiang, Henan Province 453000, China
ENT & HN Surgery Department, The First Afﬁliated Hospital of
Hainan Medical University, Haikou, Hainan Province 570102,