lncRNA HOTAIR Inhibits Mineralization in Osteoblastic Osteosarcoma Cells by Epigenetically Repressing ALPL

lncRNA HOTAIR Inhibits Mineralization in Osteoblastic Osteosarcoma Cells by Epigenetically... HOTAIR is a lncRNA that plays critical role in gene regulation and chromatin dynamics through epigenetic mechanisms. In this work we studied the physiological role of HOTAIR during the process of mineralization using osteoblastic osteosarcoma cells focusing in ALPL (Tissue Non-Specific Alkaline Phosphatase), a pivotal gene that controls bone formation. HOTAIR knockdown resulted in upregulation of ALPL, increase of alkaline phosphatase (ALP) activity, and enhanced mineralization in osteoblastic SaOS-2 cells cultured in mineralizing medium. Luciferase assays using reporter vectors containing ALPL promoter showed that HOTAIR repression increases ALPL promoter activity. Furthermore, HOTAIR knockdown increased histone H3K4 methylation levels at ALPL promoter region, suggesting that ALPL repression by HOTAIR is regulated by epigenetic mechanisms. This work supports that physiological bone formation is epigenetically regulated by a lncRNA. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Calcified Tissue International Springer Journals

lncRNA HOTAIR Inhibits Mineralization in Osteoblastic Osteosarcoma Cells by Epigenetically Repressing ALPL

Misawa, Aya; Orimo, Hideo
Calcified Tissue International, Volume 103 (4) – May 30, 2018
9 pages
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Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Life Sciences; Biochemistry, general; Endocrinology; Orthopedics; Cell Biology
ISSN
0171-967X
eISSN
1432-0827
D.O.I.
10.1007/s00223-018-0434-0
Publisher site
See Article on Publisher Site

Abstract

HOTAIR is a lncRNA that plays critical role in gene regulation and chromatin dynamics through epigenetic mechanisms. In this work we studied the physiological role of HOTAIR during the process of mineralization using osteoblastic osteosarcoma cells focusing in ALPL (Tissue Non-Specific Alkaline Phosphatase), a pivotal gene that controls bone formation. HOTAIR knockdown resulted in upregulation of ALPL, increase of alkaline phosphatase (ALP) activity, and enhanced mineralization in osteoblastic SaOS-2 cells cultured in mineralizing medium. Luciferase assays using reporter vectors containing ALPL promoter showed that HOTAIR repression increases ALPL promoter activity. Furthermore, HOTAIR knockdown increased histone H3K4 methylation levels at ALPL promoter region, suggesting that ALPL repression by HOTAIR is regulated by epigenetic mechanisms. This work supports that physiological bone formation is epigenetically regulated by a lncRNA.

Journal

Calcified Tissue International – Springer Journals

Published: May 30, 2018

References

  • Alkaline phosphatases: structure, substrate specificity and functional relatedness to other members of a large superfamily of enzymes
    Millán, JL
  • Regional assignment of the gene for human liver/bone/kidney alkaline phosphatase to chromosome 1p36.1-p34
    Smith, M; Weiss, MJ; Griffin, CA; Murray, JC; Buetow, KH; Emanuel, BS; Henthorn, PS; Harris, H
  • Structure of the human liver/bone/kidney alkaline phosphatase gene
    Weiss, MJ; Ray, K; Henthorn, PS; Lamb, B; Kadesch, T; Harris, H
  • Characterization of a 5′-flanking region of the human liver/bone/kidney alkaline phosphatase gene: two kinds of mRNA from a single gene
    Matsuura, S; Kishi, F; Kajii, T
  • The mechanism of mineralization and the role of alkaline phosphatase in health and disease
    Orimo, H
  • Hypophosphatasia; a new developmental anomaly
    Rathbun, JC
  • Hypophosphatasia: an overview of the disease and its treatment
    Bianchi, ML
  • NT5E mutations and arterial calcifications
    St Hilaire, C; Ziegler, SG; Markello, TC; Brusco, A; Groden, C; Gill, F; Carlson-Donohoe, H; Lederman, RJ; Chen, MY; Yang, D; Siegenthaler, MP; Arduino, C; Mancini, C; Freudenthal, B; Stanescu, HC; Zdebik, AA; Chaganti, RK; Nussbaum, RL; Kleta, R; Gahl, WA; Boehm, M
  • Common mutation F310L and T1559del in the tissue-nonspecific alkaline phosphatase gene are related to distinct phenotypes in Japanese patients with hypophosphatasia
    Michigami, T; Uchihashi, T; Suzuki, A; Tachikwa, K; Nakajima, S; Ozono, K
  • Pathophysiology of hypophosphatasia and the potential role of asfotase alfa
    Orimo, H
  • Hypophosphatasia: an overview for 2017
    Whyte, MP
  • The amazing osteocyte
    Bonewald, LF
  • Update of the FANTOM web resource: high resolution transcriptome of diverse cell types in mammals
    Lizio, M; Harshbarger, J; Abugessaisa, I; Noguchi, S; Kondo, A; Severin, J; Mungall, C; Arenillas, D; Mathelier, A; Medvedeva, YA; Lennartsson, A; Drabløs, F; Ramilowski, JA; Rackham, O; Gough, J; Andersson, R; Sandelin, A; Ienasescu, H; Ono, H; Bono, H; Hayashizaki, Y; Carninci, P; Forrest, AR; Kasukawa, T; Kawaji, H
  • An atlas of human long non-coding RNAs with accurate 5′ ends
    Hon, CC; Ramilowski, JA; Harshbarger, J; Bertin, N; Rackham, OJ; Gough, J; Denisenko, E; Schmeier, S; Poulsen, TM; Severin, J; Lizio, M; Kawaji, H; Kasukawa, T; Itoh, M; Burroughs, AM; Noma, S; Djebali, S; Alam, T; Medvedeva, YA; Testa, AC; Lipovich, L; Yip, CW; Abugessaisa, I; Mendez, M; Hasegawa, A; Tang, D; Lassmann, T; Heutink, P; Babina, M; Wells, CA; Kojima, S; Nakamura, Y; Suzuki, H; Daub, CO; Hoon, MJ; Arner, E; Hayashizaki, Y; Carninci, P; Forrest, AR
  • The landscape of long noncoding RNAs in the human transcriptome
    Iyer, MK; Niknafs, YS; Malik, R; Singhal, U; Sahu, A; Hosono, Y; Barrette, TR; Prensner, JR; Evans, JR; Zhao, S; Poliakov, A; Cao, X; Dhanasekaran, SM; Wu, YM; Robinson, DR; Beer, DG; Feng, FY; Iyer, HK; Chinnaiyan, AM
  • lncRNAdb v2.0: expanding the reference database for functional long noncoding RNAs
    Quek, XC; Thomson, DW; Maag, JL; Bartonicek, N; Signal, B; Clark, MB; Gloss, BS; Dinger, ME
  • The long noncoding MALAT-1 RNA indicates a poor prognosis in non-small cell lung cancer and induces migration and tumor growth
    Schmidt, LH; Spieker, T; Koschmieder, S; Schäffers, S; Humberg, J; Jungen, D; Bulk, E; Hascher, A; Wittmer, D; Marra, A; Hillejan, L; Wiebe, K; Berdel, WE; Wiewrodt, R; Muller-Tidow, C
  • Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis
    Gupta, RA; Shah, N; Wang, KC; Kim, J; Horlings, HM; Wong, DJ; Tsai, MC; Hung, T; Argani, P; Rinn, JL; Wang, Y; Brzoska, P; Kong, B; Li, R; West, RB; Vijver, MJ; Sukumar, S; Chang, HY
  • Long noncoding RNA as modular scaffold of histone modification complexes
    Tsai, MC; Manor, O; Wan, Y; Mosammaparast, N; Wang, JK; Lan, F; Shi, Y; Segal, E; Chang, HY
  • LncRNA HOTAIR: a master regulator of chromatin dynamics and cancer
    Bhan, A; Mandal, SS
  • The long non-coding HOTAIR is modulated by cyclic stretch and WNT/β-CATENIN in human aortic valve cells and is a novel repressor of calcification genes
    Carrion, K; Dyo, J; Patel, V; Sasik, R; Mohamed, SA; Hardiman, G; Nigam, V
  • Effects of phosphates on the expression of tissue-nonspecific alkaline phosphatase gene and phosphate-regulating genes in short-term cultures of human osteosarcoma cell lines
    Orimo, H; Shimada, T
  • The role of tissue-nonspecific alkaline phosphatase in the phosphate-induced activation of alkaline phosphatase and mineralization in SaOS-2 human osteoblast-like cells
    Orimo, H; Shimada, T
  • Regulation of the human tissue-nonspecific alkaline phosphatase gene expression by all-trans-retinoic acid in SaOS-2 osteosarcoma cell line
    Orimo, H; Shimada, T
  • Characterization of a human osteosarcoma cell line (Saos-2) with osteoblastic properties
    Rodan, SB; Imai, Y; Thiede, MA; Wesolowski, G; Thompson, D; Bar-Shavit, Z; Shull, S; Mann, K; Rodan, GA
  • Human osteosarcoma cells spontaneously release matrix-vesicle-like structures with the capacity to mineralize
    Fedde, KN
  • Identification of novel androgen response genes in prostate cancer cells by coupling chromatin immunoprecipitation and genomic microarray analysis
    Takayama, K; Kaneshiro, K; Tsutsumi, S; Horie-Inoue, K; Ikeda, K; Urano, T; Ijichi, N; Ouchi, Y; Shirahige, K; Aburatani, H; Inoue, S
  • Structure of the human liver/bone/kidney alkaline phosphatase gene
    Weiss
  • Analysis of the human liver/bone/kidney alkaline phosphatase promoter in vivo and in vitro
    Kiledjian
  • Transcription factor Sp3 activates the liver/bone/kidney-type alkaline phosphatase promoter in hematopoietic cells
    Yusa, N
  • Smad-interacting protein 1 is a repressor of liver/bone/kidney alkaline phosphatase transcription in bone morphogenetic protein-induced osteogenic differentiation of C2C12 cells
    Tylzanowski
  • Histone deacetylase inhibitor MS-275 stimulates bone formation in part by enhancing Dhx36-mediated TNAP transcription
    Kim, HN; Lee, JH; Bae, SC; Ryoo, HM; Kim, HH; Ha, H; Lee, ZH
  • Epigenetic regulation of alkaline phosphatase in human cells of the osteoblastic lineage
    Delgado-Calle, J; Sañudo, C; Sánchez-Verde, L; García-Renedo, RJ; Arozamena, J; Riancho, JA
  • In search of representative models of human bone-forming cells for cytocompatibility studies
    Saldana, L
  • In search of an osteoblast cell model for in vitro research
    Czekanska
  • Interaction of human osteoblast-like Saos-2 and MG-63 cells with thermally oxidized surfaces of a titanium-niobium alloy
    Vandrovcova
  • Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis
    Gupta

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