Liposome-entrapped Polymerases as Models for Microscale/Nanoscale Bioreactors

Liposome-entrapped Polymerases as Models for Microscale/Nanoscale Bioreactors J. Membrane Biol. 191, 87–97 (2003) DOI: 10.1007/s00232-002-1046-0 Topical Review P.-A. Monnard Department of Molecular Biology, Massachusetts General Hospital, Wellman 9, 50 Blossom St., Boston, MA 02114, USA Received: 5 April 2002/Revised: 14 August 2002 Introduction enzyme. Microsize vesicle-based bioreactors could be developed that combine both the properties of bio- The first enzyme-containing lipid vesicles were de- logical and nanotechnological systems. Such systems veloped soon after the pioneering work of Bangham would represent a kind of artificial minimal cell et al. (1964, 1965) that outlined the physical proper- (Pohorille & Deamer, 2002), and could be engineered ties of these lipid structures in an aqueous medium. for a specific task related to therapeutic and diag- Such systems have been envisioned as drug-delivery nostic applications. Unlike genetically modified cells vehicles for gene therapy or enzyme-replacement that require stringent conditions to survive and can therapy and as bioreactors, which could ultimately be damaged during genetic manipulations, these li- serve as microcompartments for in vitro selection of, posome-based bioreactors would only be composed e.g., catalytic RNA (ribozymes), or as blueprints for of the desired genetic material or/and metabolic ac- an artificial minimal cell. tivity, thereby overcoming safety and ethical issues Amphiphile vesicles with The Journal of Membrane Biology Springer Journals

Liposome-entrapped Polymerases as Models for Microscale/Nanoscale Bioreactors

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Copyright © 2003 by Springer-Verlag New York Inc.
Life Sciences; Biochemistry, general; Human Physiology
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