Mammalian Genome 11, 342–343 (2000). Incorporating Mouse Genome © Springer-Verlag New York Inc. 2000 1 1 2 2 1 Massoud Malek, Stefan Marklund, Cheryl Dyer, Robert Matteri, Max Rothschild Department of Animal Science, 2255H Kildee Hall, Iowa State University, Ames, Iowa, 50011, USA United States Department of Agriculture, University of Missouri, Columbia, Missouri 65211, USA Received: 25 August 1999 / Accepted: 2 December 1999 It is well established that the brain, and specifically the hypothala- panel developed by Yerle et al. (1996) was used to physically map mus, is a major site where various central nervous system signals this gene in the pig. Primers (forward 58-ACG CTG CTG CTT are integrated to affect the expression of complex hormonal and CTG CTA CT -38; and reverse 58-AGC GGG CAT CCT GAC neuroendocrine functions, such as food intake and energy homeo- CAT -38) were used that produced a 251-bp PCR product that was stasis. Orexin-A and B (also called prepro-orexin), are hypotha- then used for gene mapping. Amplified products were analyzed by lamic peptides, encoded by a single mRNA transcript, which are 2% Metaphor gel electrophoresis. derived from the same precursor. These peptides bind and activate The results for sequencing and
Mammalian Genome – Springer Journals
Published: Apr 1, 2000
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