Linkage and physical mapping of the porcine prepro-orexin gene

Linkage and physical mapping of the porcine prepro-orexin gene Mammalian Genome 11, 342–343 (2000). Incorporating Mouse Genome © Springer-Verlag New York Inc. 2000 1 1 2 2 1 Massoud Malek, Stefan Marklund, Cheryl Dyer, Robert Matteri, Max Rothschild Department of Animal Science, 2255H Kildee Hall, Iowa State University, Ames, Iowa, 50011, USA United States Department of Agriculture, University of Missouri, Columbia, Missouri 65211, USA Received: 25 August 1999 / Accepted: 2 December 1999 It is well established that the brain, and specifically the hypothala- panel developed by Yerle et al. (1996) was used to physically map mus, is a major site where various central nervous system signals this gene in the pig. Primers (forward 58-ACG CTG CTG CTT are integrated to affect the expression of complex hormonal and CTG CTA CT -38; and reverse 58-AGC GGG CAT CCT GAC neuroendocrine functions, such as food intake and energy homeo- CAT -38) were used that produced a 251-bp PCR product that was stasis. Orexin-A and B (also called prepro-orexin), are hypotha- then used for gene mapping. Amplified products were analyzed by lamic peptides, encoded by a single mRNA transcript, which are 2% Metaphor gel electrophoresis. derived from the same precursor. These peptides bind and activate The results for sequencing and http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mammalian Genome Springer Journals

Linkage and physical mapping of the porcine prepro-orexin gene

Loading next page...
 
/lp/springer_journal/linkage-and-physical-mapping-of-the-porcine-prepro-orexin-gene-MHw7b00381
Publisher
Springer Journals
Copyright
Copyright © 2000 by Springer-Verlag New York Inc.
Subject
Life Sciences; Cell Biology; Animal Genetics and Genomics; Human Genetics
ISSN
0938-8990
eISSN
1432-1777
D.O.I.
10.1007/s003350010064
Publisher site
See Article on Publisher Site

Abstract

Mammalian Genome 11, 342–343 (2000). Incorporating Mouse Genome © Springer-Verlag New York Inc. 2000 1 1 2 2 1 Massoud Malek, Stefan Marklund, Cheryl Dyer, Robert Matteri, Max Rothschild Department of Animal Science, 2255H Kildee Hall, Iowa State University, Ames, Iowa, 50011, USA United States Department of Agriculture, University of Missouri, Columbia, Missouri 65211, USA Received: 25 August 1999 / Accepted: 2 December 1999 It is well established that the brain, and specifically the hypothala- panel developed by Yerle et al. (1996) was used to physically map mus, is a major site where various central nervous system signals this gene in the pig. Primers (forward 58-ACG CTG CTG CTT are integrated to affect the expression of complex hormonal and CTG CTA CT -38; and reverse 58-AGC GGG CAT CCT GAC neuroendocrine functions, such as food intake and energy homeo- CAT -38) were used that produced a 251-bp PCR product that was stasis. Orexin-A and B (also called prepro-orexin), are hypotha- then used for gene mapping. Amplified products were analyzed by lamic peptides, encoded by a single mRNA transcript, which are 2% Metaphor gel electrophoresis. derived from the same precursor. These peptides bind and activate The results for sequencing and

Journal

Mammalian GenomeSpringer Journals

Published: Apr 1, 2000

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off