Mammalian Genome 10, 405–409 (1999). Incorporating Mouse Genome © Springer-Verlag New York Inc. 1999 Linkage and physical mapping of rat microsatellites derived from minisatellite loci 1 2 1 3 3 Fabienne Giraudeau, Franc ¸oise Apiou, Vale ´rie Amarger, Pamela J. Kaisaki, Marie-The ´re `se Bihoreau, 3 1,4 3 Mark Lathrop, Gilles Vergnaud, Dominique Gauguier Institut de Ge ´ne ´tique et Microbiologie, Universite ´ Paris-Sud, France Institut Curie-CNRS UMR 147, 26 rue d’Ulm, Paris, France The Wellcome Trust Centre for Human Genetics, University of Oxford, Windmill Road, Headington, Oxford OX3 7BN, UK Centre d’Etudes du Bouchet, BP3, 91710 Vert le Petit, France Received: 16 October 1998 / Accepted: 24 November 1998 Microsatellite and minisatellite markers are the major components Biosciences Inc., Plymouth, MN), and oligonucleotides were ob- of genetic linkage maps. Microsatellite markers are spread along tained from Genosys Biotechnologies (Pampisford, UK). the genome, whereas minisatellites show a non-random chromo- We were unable to design PCR primers in the sequence of 22 somal distribution, different from species to species. In human, clones owing to either the poor quality of the sequence or the they are predominantly clustered in the subtelomeric regions, de- length or complexity of the repeated element. The
Mammalian Genome – Springer Journals
Published: Apr 1, 1999
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