It has been established that, under light, acute decrease in succinate dehydrogenase (SDH) activity in maize (Zea mays L.) leaves occurs, which is likely due to lowering the role of tricarboxylic acid cycle (TCA) as energy supplier under actively functioning photosynthesis. The mechanism underlying the light regulation of SDH subunit B genes transcription level was clarified. It was shown that, in green maize leaves, mRNA of only gene sdh2-3 encoding large subunit of this enzyme was detected. The apparent absence of transcripts of genes sdh2-1 and sdh2-2 encoding other isoforms of the large subunit of the enzyme is explained by their differential expression on other stages of plant ontogenesis due to metabolism restructuring for triggering photosynthesis operation. It was found that the active form of phytochrome A participates in the regulation of gene sdh2-3 expression, leading to the decrease in the amount of mRNA of the given gene in leaves. Application of specific inhibitor of cell membranes calcium channels (ruthenium red) and EGTA allowed us to reveal that the change in calcium concentration in the nucleus is due to its redistribution between cell compartments, in particular between the nucleus and cytoplasm. In addition, it was found that expression of gene pif3 encoding transcription factor PIF3 is changed in a light-dependent manner, in dependency on the state of the phytochrome system, and this correlates with gene sdh2-3 functioning. These findings may indicate the phytochrome-mediated mechanism of the regulation of this gene with participation in the process of transcription factor PIF3.
Russian Journal of Plant Physiology – Springer Journals
Published: Jun 24, 2016
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera