Lead Neurotoxicity on Human Neuroblastoma Cell Line SH-SY5Y is Mediated via Transcription Factor EGR1/Zif268 Induced Disrupted in Scherophernia-1 Activation

Lead Neurotoxicity on Human Neuroblastoma Cell Line SH-SY5Y is Mediated via Transcription Factor... 2+ 2+ Lead (Pb ) is a well-known type of neurotoxin and chronic exposure to Pb induces cognition dysfunction. In this work, 2+ the potential role of early growth response gene 1 (EGR1) in the linkage of Pb exposure and disrupted in scherophernia-1 (DISC1) activity was investigated. Human neuroblastoma cell line SH-SY5Y was subjected to different concentrations of 2+ lead acetate (PbAc) to determine the effect of Pb exposure on the cell viability, apoptosis, and activity of EGR1 and DISC1. Then the expression of EGR1 in SH-SY5Y cells was knocked down with specific siRNA to assess the function of EGR1 in 2+ Pb induced activation of DISC1. The interaction between EGR1 and DISC1 was further validated with dual luciferase assay, Supershift electrophoretic mobility shift assay (EMSA), and chromatin immunoprecipitation (ChIP)-PCR. Administration of PbAc decreased cell viability and induced apoptosis in SH-SY5Y cells in a dose-dependent manner. Additionally, exposure to PbAc also up-regulated expression of EGR1 and DISC1 at all concentrations. Knockdown of EGR1 blocked the effect 2+ of PbAc on SH-SY5Y cells, indicating the central role of EGR1 in the function of Pb on activity of DISC1. Based on the 2+ results of dual luciferase assay, Supershift EMSA, and http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Neurochemical Research Springer Journals

Lead Neurotoxicity on Human Neuroblastoma Cell Line SH-SY5Y is Mediated via Transcription Factor EGR1/Zif268 Induced Disrupted in Scherophernia-1 Activation

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Publisher
Springer US
Copyright
Copyright © 2018 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Biomedicine; Neurosciences; Neurochemistry; Biochemistry, general; Cell Biology; Neurology
ISSN
0364-3190
eISSN
1573-6903
D.O.I.
10.1007/s11064-018-2539-2
Publisher site
See Article on Publisher Site

Abstract

2+ 2+ Lead (Pb ) is a well-known type of neurotoxin and chronic exposure to Pb induces cognition dysfunction. In this work, 2+ the potential role of early growth response gene 1 (EGR1) in the linkage of Pb exposure and disrupted in scherophernia-1 (DISC1) activity was investigated. Human neuroblastoma cell line SH-SY5Y was subjected to different concentrations of 2+ lead acetate (PbAc) to determine the effect of Pb exposure on the cell viability, apoptosis, and activity of EGR1 and DISC1. Then the expression of EGR1 in SH-SY5Y cells was knocked down with specific siRNA to assess the function of EGR1 in 2+ Pb induced activation of DISC1. The interaction between EGR1 and DISC1 was further validated with dual luciferase assay, Supershift electrophoretic mobility shift assay (EMSA), and chromatin immunoprecipitation (ChIP)-PCR. Administration of PbAc decreased cell viability and induced apoptosis in SH-SY5Y cells in a dose-dependent manner. Additionally, exposure to PbAc also up-regulated expression of EGR1 and DISC1 at all concentrations. Knockdown of EGR1 blocked the effect 2+ of PbAc on SH-SY5Y cells, indicating the central role of EGR1 in the function of Pb on activity of DISC1. Based on the 2+ results of dual luciferase assay, Supershift EMSA, and

Journal

Neurochemical ResearchSpringer Journals

Published: Jun 4, 2018

References

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