Large-scale codon de-optimisation of the p29 replicase gene by synonymous substitutions causes a loss of infectivity of melon necrotic spot virus

Large-scale codon de-optimisation of the p29 replicase gene by synonymous substitutions causes a... The effect of synonymous substitutions in the melon necrotic spot virus p29 replicase gene on viral pathogenicity was investigated. The codons in the p29 gene were replaced by the least frequently used synonymous codons in Arabidopsis thaliana or melons. Mechanical inoculation of melon with p29 variants resulted in a loss of viral infectivity when all, one-half, or one-quarter of the gene was de-optimised. The effect of the de-optimisation in one-sixth of the gene was different depending on the de-optimised region. These results demonstrate that large-scale codon bias de-optimisation without amino acid substitutions of the p29 gene alter viral infectivity. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Large-scale codon de-optimisation of the p29 replicase gene by synonymous substitutions causes a loss of infectivity of melon necrotic spot virus

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Publisher
Springer Vienna
Copyright
Copyright © 2013 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1683-x
Publisher site
See Article on Publisher Site

Abstract

The effect of synonymous substitutions in the melon necrotic spot virus p29 replicase gene on viral pathogenicity was investigated. The codons in the p29 gene were replaced by the least frequently used synonymous codons in Arabidopsis thaliana or melons. Mechanical inoculation of melon with p29 variants resulted in a loss of viral infectivity when all, one-half, or one-quarter of the gene was de-optimised. The effect of the de-optimisation in one-sixth of the gene was different depending on the de-optimised region. These results demonstrate that large-scale codon bias de-optimisation without amino acid substitutions of the p29 gene alter viral infectivity.

Journal

Archives of VirologySpringer Journals

Published: Sep 1, 2013

References

  • Codon usage bias amongst plant viruses
    Adams, MJ; Antoniw, JF
  • Watermelon necrosis caused by a strain of Melon necrotic spot virus
    Avgelis, A
  • Live attenuated influenza virus vaccines by computer-aided rational design
    Mueller, S; Coleman, JR; Papamichail, D; Ward, CB; Nimnual, A; Futcher, B; Skiena, S; Wimmer, E
  • Heterogeneity in codon usages of sobemovirus genes
    Zhou, H; Wang, H; Huang, LF; Naylor, M; Clifford, P

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