Arch Virol (2000) 145: 2173–2182
Lack of disease-speciﬁc amino acid changes in the viral proteins
of JC virus isolates from the brain with progressive
, C. Sugimoto
, H.-Y. Zheng
, T. Kitamura
, and Y. Yogo
Department of Urology, Faculty of Medicine, The University of Tokyo, Tokyo, Japan
Department of Viral Infection, The Institute of Medical Science,
The University of Tokyo, Tokyo, Japan
Accepted May 22, 2000
Summary. Progressive multifocal leukoencephalopathy (PML) is a fatal de-
myelinating disease of the central nervous system caused by a ubiquitous human
polyomavirus designated as JC virus (JCV). JCVs in the brain of PML patients
(PML-type JCVs) contain various regulatory regions generated from the archety-
pal regulatory region during persistence in the patients. We determined the com-
plete DNA sequences of 2 PML-type isolates and 5 archetype isolates. Amino
acid sequences of individual viral proteins were deduced from complete DNA se-
quences, and were compared among 16 isolates (6 PML types and 10 archetypes).
From the data obtained, we concluded that PML-associated amino acid changes
did not occur in the viral proteins of PML-type JCVs.
Human polyomavirus JC virus (JCV) causes a fatal demyelinating disease of the
central nervous system, known as progressive multifocal leukoencephalopathy
(PML), in patients with decreased immune competence . This virus, however,
is widespread in human populations. Most individuals are asymptomatically in-
fected with JCV during childhood [27, 28]. After primary infection, JCV persists
in the renal tissue throughout life [8, 20, 33].
The DNA sequence data reported here have been deposited in the GSDB, DDBJ,
EMBL, and NCBI nucleotide sequence databases with accession numbers AB038249 (CY),
1), and AB038255 (Tky-2a).