Biotechnol Lett https://doi.org/10.1007/s10529-018-2581-9 ORIGINAL RESEARCH PAPER Labeling of endothelial cells with magnetic microbeads by angiophagy . . . . Jessica Thomas Desiree Jones Leni Moldovan Mirela Anghelina Keith J. Gooch Nicanor I. Moldovan Received: 3 March 2018 / Accepted: 28 May 2018 Springer Science+Business Media B.V., part of Springer Nature 2018 Abstract was conﬁrmed by (i) increased cell granularity Objectives Attachment of magnetic particles to cells assessed by ﬂow cytometry, and (ii) the presence of is needed for a variety of applications but is not always an F-actin rich layer around many of the intracellular possible or efﬁcient. Simpler and more convenient beads, visualized by confocal microscopy. For con- methods are thus desirable. In this study, we tested the ﬂuent cultures, the average number of beads per cell hypothesis that endothelial cells (EC) can be loaded was 4.4 and 4.2, with and without the presence of the with micron-size magnetic beads by the phagocytosis- anti-VEGFR2 antibody, respectively. However, while like mechanism ‘angiophagy’. To this end, human the actively dividing cells took up 2.9 unconjugated umbilical vein EC (HUVEC) were incubated with beads on average, this number increased to 5.2 if magnetic beads conjugated or not (control) with an binding
Biotechnology Letters – Springer Journals
Published: Jun 6, 2018
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