Access the full text.
Sign up today, get DeepDyve free for 14 days.
M. Hugosson, D. Andreu, H. Boman, E. Glaser (1994)
Antibacterial peptides and mitochondrial presequences affect mitochondrial coupling, respiration and protein import.European journal of biochemistry, 223 3
K. Künkele, P. Juin, Carole Pompa, F. Nargang, J. Henry, W. Neupert, R. Lill, M. Thieffry (1998)
The Isolated Complex of the Translocase of the Outer Membrane of MitochondriaThe Journal of Biological Chemistry, 273
D. Cyr, M. Douglas (1991)
Early events in the transport of proteins into mitochondria. Import competition by a mitochondrial presequence.The Journal of biological chemistry, 266 32
D. Roise (1997)
Recognition and Binding of Mitochondrial Presequences during the Import of Proteins into MitochondriaJournal of Bioenergetics and Biomembranes, 29
N. Pfanner, Harald Müller, M. Harmey, Walter Neupert (1987)
Mitochondrial protein import: involvement of the mature part of a cleavable precursor protein in the binding to receptor sites.The EMBO Journal, 6
S. Glaser, M. Cumsky (1990)
A synthetic presequence reversibly inhibits protein import into yeast mitochondria.The Journal of biological chemistry, 265 15
B. Glick (1995)
Can Hsp70 proteins act as force-generating motors?Cell, 80
D. Rapaport, W. Neupert, R. Lill (1997)
Mitochondrial Protein ImportThe Journal of Biological Chemistry, 272
W. Voos, H. Martin, T. Krimmer, N. Pfanner (1999)
Mechanisms of protein translocation into mitochondria.Biochimica et biophysica acta, 1422 3
D. Maćasev, E. Newbigin, J. Whelan, T. Lithgow (2000)
How do plant mitochondria avoid importing chloroplast proteins? Components of the import apparatus Tom20 and Tom22 from Arabidopsis differ from their fungal counterparts.Plant physiology, 123 3
E. Glaser, S. Sjöling, M. Tanudji, J. Whelan (1998)
Mitochondrial protein import in plants – Signals, Sorting, Targeting, Processing and RegulationPlant Molecular Biology, 38
Elzbieta Glaser, Carina Knorpp, M. Hugosson, Erik Stedingk (1995)
Macromolecular movement into mitochondria.Methods in cell biology, 50
B. Merrifield (1985)
Solid phase synthesisBioscience Reports, 5
W. Neupert (1997)
Protein import into mitochondria.Annual review of biochemistry, 66
X. Zhang, A. Elofsson, D. Andreu, E. Glaser (1999)
Interaction of mitochondrial presequences with DnaK and mitochondrial hsp70.Journal of molecular biology, 288 1
K. Hill, K. Model, M. Ryan, K. Dietmeier, F. Martin, R. Wagner, N. Pfanner (1998)
Tom40 forms the hydrophilic channel of the mitochondrial import pore for preproteinsNature, 395
S. Sjöling, E. Glaser (1998)
Mitochondrial targeting peptides in plantsTrends in Plant Science, 3
L. Jänsch, V. Kruft, U. Schmitz, H. Braun (1998)
Unique Composition of the Preprotein Translocase of the Outer Mitochondrial Membrane from Plants*The Journal of Biological Chemistry, 273
D. Rapaport, W. Neupert, R. Lill (1997)
Mitochondrial protein import. Tom40 plays a major role in targeting and transloca-tion of preproteins by forming a specific binding site for the presequenceJ. Biol. Chem., 272
J. Rassow, B. Guiard, U. Wienhues, V. Herzog, F. Hartl, W. Neupert (1989)
Translocation arrest by reversible folding of a precursor protein imported into mitochondria. A means to quantitate translocation contact sitesThe Journal of Cell Biology, 109
D. Leuenberger, Nikolaus Bally, G. Schatz, C. Koehler (1999)
Different import pathways through the mitochondrial intermembrane space for inner membrane proteinsThe EMBO Journal, 18
Ronald Emaus, Ron Grunwald, J. Lemasters (1986)
Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties.Biochimica et biophysica acta, 850 3
Jörg Martin, Kerstin Mahlke, Nikolaus PfannerS (1991)
Role of an energized inner membrane in mitochondrial protein import. Delta psi drives the movement of presequences.The Journal of biological chemistry, 266 27
C. Voisine, E. Craig, N. Zufall, O. Ahsen, N. Pfanner, W. Voos (1999)
The Protein Import Motor of Mitochondria Unfolding and Trapping of Preproteins Are Distinct and Separable Functions of Matrix Hsp70Cell, 97
G. Schatz (1997)
Just follow the acid chainNature, 388
E. Stedingk, P. Pavlov, V. Grinkevich, E. Glaser (1997)
Mitochondrial protein import: modification of sulfhydryl groups of the inner mitochondrial membrane import machinery in Solanum tuberosum inhibits protein importPlant Molecular Biology, 35
F. Moro, C. Sirrenberg, H. Schneider, W. Neupert, M. Brunner (1999)
The TIM17·23 preprotein translocase of mitochondria: composition and function in protein transport into the matrixThe EMBO Journal, 18
K. Künkele, Susanne Heins, M. Dembowski, F. Nargang, R. Benz, M. Thieffry, J. Walz, R. Lill, S. Nussberger, W. Neupert (1998)
The Preprotein Translocation Channel of the Outer Membrane of MitochondriaCell, 93
Bastian Feifel, H. Schönfeld, P. Christen (1998)
d-Peptide Ligands for the Co-chaperone DnaJ*The Journal of Biological Chemistry, 273
M. Boutry, Nam-Hai Chua (1985)
A nuclear gene encoding the beta subunit of the mitochondrial ATP synthase in Nicotiana plumbaginifolia.The EMBO Journal, 4
S. Rüdiger, A. Buchberger, B. Bukau (1997)
Interaction of Hsp70 chaperones with substratesNature Structural Biology, 4
Y. Abe, Toshihiro Shodai, T. Muto, K. Mihara, H. Torii, S. Nishikawa, T. Endo, D. Kohda (2000)
Structural Basis of Presequence Recognition by the Mitochondrial Protein Import Receptor Tom20Cell, 100
P. Dessi, C. Rudhe, E. Glaser (2000)
Studies on the topology of the protein import channel in relation to the plant mitochon-drial processing peptidase integrated into the bc 1 complex. 2000Plant J., 24
(1992)
The regulation of mitochondrial respiratory activity: the roles of adenylate synthase and the ATP/ADP translocator
D. Roise, S. Horvath, J. Tomich, J. Richards, G. Schatz (1986)
A chemically synthesized pre‐sequence of an imported mitochondrial protein can form an amphiphilic helix and perturb natural and artificial phospholipid bilayers.The EMBO Journal, 5
M. Maduke, D. Roise (1993)
Import of a mitochondrial presequence into protein-free phospholipid vesiclesScience, 260
M. Demura, N. Kamo, Y. Kobatake (1987)
Mitochondrial membrane potential estimated with the correction of probe binding.Biochimica et biophysica acta, 894 3
V. Hines, A. Brandt, G. Griffiths, H. Horstmann, H. Brütsch, Gottfried Schatz (1990)
Protein import into yeast mitochondria is accelerated by the outer membrane protein MAS70.The EMBO Journal, 9
S. Glaser, M. Cumsky (1990)
Localization of a synthetic presequence that blocks protein import into mitochondria.The Journal of biological chemistry, 265 15
M. Bauer, S. Hofmann, W. Neupert, M. Brunner (2000)
Protein translocation into mitochondria: the role of TIM complexes.Trends in cell biology, 10 1
S. Rüdiger, L. Germeroth, J. Schneider-Mergener, B. Bukau (1997)
Substrate specificity of the DnaK chaperone determined by screening cellulose‐bound peptide librariesThe EMBO Journal, 16
G. Heijne (1986)
Mitochondrial targeting sequences may form amphiphilic helices.The EMBO journal, 5 6
A.-C. Fricaud, D.G. Whitehouse, A.L. Moore (1992)
Moledular, Biochemical and Physiolog-ical Aspects of Plant Respiration
K.P. Kunkele, S. Heins, M Dembowski, F.E. Nargang, R. Benz, M. Thieffry, J. Walz, R. Lill, S. Nussberger, W. Neu-pert (1998)
The isolated complex of the translocase of the outer membrane of mitochondria. Characterization of the cation-selective and voltage-gated preprotein-conducting poreCell, 93
S. Rüdiger, L. Germeroth, J. Schneider-Mergener, B. Bukau (1997)
Interaction of Hsp70 chaperones with substratesEMBO J., 16
M. Bradford (1976)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical biochemistry, 72
D. Cyr, R. Stuart, W. Neupert (1993)
A matrix ATP requirement for presequence translocation across the inner membrane of mitochondria.The Journal of biological chemistry, 268 32
E. Glaser, S. Sjöling, M. Tanudji, J. Whelan (1998)
Mitochondrial protein import in plants
J. Whelan, M. Hugosson, E. Glaser, D. Day (1995)
Studies on the import and processing of the alternative oxidase precursor by isolated soybean mitochondriaPlant Molecular Biology, 27
C. Ungermann, B. Guiard, W. Neupert, D. Cyr (1996)
The delta psi‐ and Hsp70/MIM44‐dependent reaction cycle driving early steps of protein import into mitochondria.The EMBO Journal, 15
S. Wilpe, M. Ryan, K. Hill, A. Maarse, C. Meisinger, J. Brix, P. Dekker, M. Moczko, R. Wagner, M. Meijer, B. Guiard, A. Hönlinger, N. Pfanner (1999)
Tom22 is a multifunctional organizer of the mitochondrial preprotein translocaseNature, 401
P. Dessi, M. Smith, D. Day, J. Whelan (1996)
Characterization of the import pathway of the F(A)d subunit of mitochondrial ATP synthase into isolated plant mitochondria.Archives of biochemistry and biophysics, 335 2
R. Scaduto, L. Grotyohann (1999)
Measurement of mitochondrial membrane potential using fluorescent rhodamine derivatives.Biophysical journal, 76 1 Pt 1
G. Schatz, B. Dobberstein (1996)
Common Principles of Protein Translocation Across MembranesScience, 271
J. Duszyński, K. Bogucka, L. Wojtczak (1984)
Homeostasis of the protonmotive force in phosphorylating mitochondria.Biochimica et biophysica acta, 767 3
U. Laemmli (1970)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 227
C. Knorpp, C. Szigyarto, E. Glaser (1995)
Evidence for a novel ATP-dependent membrane-associated protease in spinach leaf mitochondria.The Biochemical journal, 310 ( Pt 2)
F. Chaumont, V. O'Riordan, M. Boutry (1990)
Protein transport into mitochondria is conserved between plant and yeast species.The Journal of biological chemistry, 265 28
J. Brix, S. Rüdiger, B. Bukau, J. Schneider-Mergener, N. Pfanner (1999)
Distribution of Binding Sequences for the Mitochondrial Import Receptors Tom20, Tom22, and Tom70 in a Presequence-carrying Preprotein and a Non-cleavable Preprotein*The Journal of Biological Chemistry, 274
P. Dessi, Charlotta Rudhe, Elzbieta Glaser (2000)
Studies on the topology of the protein import channel in relation to the plant mitochondrial processing peptidase integrated into the cytochrome bc1 complex.The Plant journal : for cell and molecular biology, 24 5
M. Horst, A. Azem, G. Schatz, B. Glick (1997)
What is the driving force for protein import into mitochondria?Biochimica et biophysica acta, 1318 1-2
We investigated the effect of L and D enantiomers of a 25-residue peptide derived from the N-terminal region of the presequence of Nicotiana plumbaginifolia F1β subunit of the ATP synthase, pF1β(1, 25), on import into spinach leaf mitochondria. Three in vitro synthesized precursor proteins using different import pathways were used. Import of the precursor proteins of F1β subunit of the ATP synthase, pre-F1β, and the alternative oxidase, pre-AOX, required addition of external ATP, whereas the chimeric precursor containing the N-terminal 84 amino acids of the cytochrome b 2 precursor protein linked to dihydrofolate reductase, pre-b 2(1, 84)-DHFR was not dependent on ATP. Import of pre-F1β, and pre-AOX was inhibited already at 1 μM and 3 μM concentration of the L and D enantiomers, whereas inhibition of import of pre-b 2(1, 84)-DHFR, occurred at concentrations >10 μM of both enantiomers. Binding efficiency of the precursor proteins was not affected by addition of the L and D enantiomers. There was no correlation between inhibition of import of pre-F1β and pre-AOX and dissipation of membrane potential measured as a decrease of Rhodamine 123 fluorescence quenching. The inhibitory effect of the L and D presequence enantiomers on import of pre-F1β and pre-AOX was concluded to occur within the outer membrane translocase machinery beyond the initial precursor receptor interaction. Furthermore, the fact that the D enantiomer had the same effect as the natural peptide showed that interaction of the presequence with the import machinery was not dependent on chiral properties of the presequence.
Plant Molecular Biology – Springer Journals
Published: Oct 3, 2004
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.