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P. Lauf, J. Bauer, N. Adragna, H. Fujise, A. Zade-Oppen, Kyoo Ryu, E. Delpire (1992)
Erythrocyte K-Cl cotransport: properties and regulation.The American journal of physiology, 263 5 Pt 1
J. Payne (1997)
Functional characterization of the neuronal-specific K-Cl cotransporter: implications for [K+]oregulation.American journal of physiology. Cell physiology, 273 5
J. Payne, Tamara Stevenson, L. Donaldson (1996)
Molecular Characterization of a Putative K-Cl Cotransporter in Rat BrainThe Journal of Biological Chemistry, 271
D. Kaji (1986)
Volume-sensitive K transport in human erythrocytesThe Journal of General Physiology, 88
D. Kaji, T. Kahn (1985)
Kinetics of Cl-dependent K influx in human erythrocytes with and without external Na: effect of NEM.The American journal of physiology, 249 5 Pt 1
John Payne (1997)
Functional characterization of the neuronal-specific K-Cl cotransporter: implications for [K+]o regulation.The American journal of physiology, 273 5
I. Bize, P. Muñoz, M. Canessa, P. Dunham (1998)
Stimulation of membrane serine-threonine phosphatase in erythrocytes by hydrogen peroxide and staurosporine.American journal of physiology. Cell physiology, 274 2
S. Orlov, J. Tremblay, P. Hamet (1996)
Bumetanide-sensitive Ion Fluxes in Vascular Smooth Muscle Cells: Lack of Functional Na+, K+, 2 Cl− CotransportThe Journal of Membrane Biology, 153
(1996)
Sodium transport in erythrocytes of frogRana ridibundaandRana temporaria
N. Agalakova, A. Lapin, G. Gusev (1997)
Temperature effects on ion transport across the erythrocyte membrane of the frog Rana temporaria.Comparative biochemistry and physiology. Part A, Physiology, 117 3
E. Delpire, P. Lauf (1991)
Trans effects of cellular K and Cl on ouabain-resistant Rb(K) influx in low K sheep red blood cells: further evidence for asymmetry of K-Cl cotransport [corrected].Pflugers Archiv : European journal of physiology, 419 5
P. Lauf (1983)
Thiol-dependent passive K+-Cl- transport in sheep red blood cells. V. Dependence on metabolism.The American journal of physiology, 245 5 Pt 1
M. Berenbrink, Y. Weaver, A. Cossins (1997)
Defining the volume dependence of multiple K flux pathways of trout red blood cells.The American journal of physiology, 272 4 Pt 1
J. Ellory, M. Wolowyk (1991)
Evidence for bumetanide-sensitive, Na(+)-dependent, partial Na-K-Cl co-transport in red blood cells of a primitive fish.Canadian journal of physiology and pharmacology, 69 5
Eric Delpire, P. Lauf (1991)
Kinetics of Cl-dependent K fluxes in hyposmotically swollen low K sheep erythrocytesThe Journal of General Physiology, 97
D. Kaji (1989)
Kinetics of volume-sensitive K transport in human erythrocytes: evidence for asymmetry.The American journal of physiology, 256 6 Pt 1
S. Kelley, Philip Dunham (1996)
Mechanism of swelling activation of K-Cl cotransport in inside-out vesicles of LK sheep erythrocyte membranes.The American journal of physiology, 270 4 Pt 1
C. Gillen, S. Brill, J. Payne, B. Forbush (1996)
Molecular Cloning and Functional Expression of the K-Cl Cotransporter from Rabbit, Rat, and HumanThe Journal of Biological Chemistry, 271
H. Kim, S. Sergeant, L. Forte, D. Sohn, J. Im (1989)
Activation of a Cl-dependent K flux by cAMP in pig red cells.The American journal of physiology, 256 4 Pt 1
H. Guizouarn, B. Harvey, F. Borgese, N. Gabillat, F. Garcia-Romeu, R. Motais (1993)
Volume‐activated Cl(‐)‐independent and Cl(‐)‐dependent K+ pathways in trout red blood cells.The Journal of Physiology, 462
(1997)
Volume regulation
(1991)
Evidence for bumetanidesensitive
In frog red blood cells, K-Cl cotransport (i.e., the difference between ouabain-resistant K fluxes in Cl and NO3) has been shown to mediate a large fraction of the total K+ transport. In the present study, Cl−-dependent and Cl−-independent K+ fluxes via frog erythrocyte membranes were investigated as a function of external and internal K+ ([K+] e and [K+] i ) concentration. The dependence of ouabain-resistant Cl−-dependent K+ (86Rb) influx on [K+] e over the range 0–20 mm fitted the Michaelis-Menten equation, with an apparent affinity (K m ) of 8.2 ± 1.3 mm and maximal velocity (V max ) of 10.4 ± 1.6 mmol/l cells/hr under isotonic conditions. Hypotonic stimulation of the Cl−-dependent K+ influx increased both K m (12.8 ± 1.7 mm, P < 0.05) and V max (20.2 ± 2.9 mmol/l/hr, P < 0.001). Raising [K+] e above 20 mm in isotonic media significantly reduced the Cl−-dependent K+ influx due to a reciprocal decrease of the external Na+ ([Na+] e ) concentration below 50 mm. Replacing [Na+] e by NMDG+ markedly decreased V max (3.2 ± 0.7 mmol/l/hr, P < 0.001) and increased K m (15.7 ± 2.1 mm, P < 0.03) of Cl−-dependent K+ influx. Moreover, NMDG+ Cl substitution for NaCl in isotonic and hypotonic media containing 10 mm RbCl significantly reduced both Rb+ uptake and K+ loss from red cells. Cell swelling did not affect the Na+-dependent changes in Rb+ uptake and K+ loss. In a nominally K+(Rb+)-free medium, net K+ loss was reduced after lowering [Na+] e below 50 mm. These results indicate that over 50 mm [Na+] e is required for complete activation of the K-Cl cotransporter. In nystatin-pretreated cells with various intracellular K+, Cl−-dependent K+ loss in K+-free media was a linear function of [K+] i , with a rate constant of 0.11 ± 0.01 and 0.18 ± 0.008 hr−1 (P < 0.001) in isotonic and hypotonic media, respectively. Thus K-Cl cotransport in frog erythrocytes exhibits a strong asymmetry with respect to transported K+ ions. The residual, ouabain-resistant K+ fluxes in NO3 were only 5–10% of the total and were well fitted to linear regressions. The rate constants for the residual influxes were not different from those for K+ effluxes in isotonic (∼0.014 hr−1) and hypotonic (∼0.022 hr−1) media, but cell swelling resulted in a significant increase in the rate constants.
The Journal of Membrane Biology – Springer Journals
Published: Dec 1, 1999
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