The inside-out vesicles of plasma membranes were isolated from pumpkin stem cells, and the kinetics of sucrose efflux induced by the K+-diffusion potential (ΔψD) was studied by measuring light transmission. Two phases differing in their rates and duration were identified in ΔψD-dependent changes of light transmission. The increase in ΔψDelevated the rate and magnitude of the fast phase in light transmission changes but did not markedly affect the rate of the slow phase. These two phases also differed in their sensitivity to inhibitors and to changes in sucrose concentration in the external medium. Measurements of ΔψDduring sucrose transport by means of the fluorescence probe dis-C3-(5) revealed differences in the magnitude of ΔψDand its stability in vesicles loaded with sucrose and mannitol, as well as under the action of inhibitors. The two-phase dependence of sucrose efflux from vesicles on the applied diffusion potential is discussed in the context of modern concepts on the functioning of sucrose carriers in the membranes.
Russian Journal of Plant Physiology – Springer Journals
Published: Oct 10, 2004
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