Keratinocytes seeded fibrin micro-carriers reconstitute an epidermis in full thickness wounds

Keratinocytes seeded fibrin micro-carriers reconstitute an epidermis in full thickness wounds Following the advantages of single cell transplantation in comparison to sheet grafts we improved a spinning culture system for keratinocytes on completely biodegradable micro-carriers. Since previous in vivo investigations with different carrier materials (Cytodex® and PLGA) (Voigt et al. Tissue Eng 8(2):263–272, 2002 ; Voigt et al. Tissue Eng 5:563–572, 1999 ) revealed severe cellular inflammatory reactions and persistent remnants of micro-carriers in wounds, the aim of this study was to find a completely biodegradable, physiological and non-toxic material for new micro-carriers. We succeeded in setting up a keratinocyte spinning culture system using fibrin carriers. We were able to reconstitute the epidermis in full thickness wounds in an athymic nude mouse model and in an immunocompetent porcine wound model. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Plastic Surgery Springer Journals

Keratinocytes seeded fibrin micro-carriers reconstitute an epidermis in full thickness wounds

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Publisher
Springer-Verlag
Copyright
Copyright © 2006 by Springer-Verlag
Subject
Medicine & Public Health; Plastic Surgery
ISSN
0930-343X
eISSN
1435-0130
D.O.I.
10.1007/s00238-006-0083-9
Publisher site
See Article on Publisher Site

Abstract

Following the advantages of single cell transplantation in comparison to sheet grafts we improved a spinning culture system for keratinocytes on completely biodegradable micro-carriers. Since previous in vivo investigations with different carrier materials (Cytodex® and PLGA) (Voigt et al. Tissue Eng 8(2):263–272, 2002 ; Voigt et al. Tissue Eng 5:563–572, 1999 ) revealed severe cellular inflammatory reactions and persistent remnants of micro-carriers in wounds, the aim of this study was to find a completely biodegradable, physiological and non-toxic material for new micro-carriers. We succeeded in setting up a keratinocyte spinning culture system using fibrin carriers. We were able to reconstitute the epidermis in full thickness wounds in an athymic nude mouse model and in an immunocompetent porcine wound model.

Journal

European Journal of Plastic SurgerySpringer Journals

Published: Dec 1, 2006

References

  • Skin tissue engineering
    Bannasch, H; Föhn, M; Unterberg, T; Bach, AD; Weyand, B; Stark, GB
  • Alteration of the extracellular matrix of cultured human keratinocytes by transformation and during differentiation
    Brown, KW; Parkinson, EK

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