Mammalian Genome 8, Brief Data Reports 701 GAAGCCAACC-3') to type the polymorphism by PCR with stan- dard buffers (1.5 rnM MgC12) and an annealing temperature of 55~ For Khk, primers KhkM4 (5'-dTGAGGGGCTTGTA- CAGTCGAG-3') and KhkR9 (5'-dCCACCTGGCACCC- GAATCTC-3'), designed from the sequences of rat  and mouse D5Bir4  Khk cDNA, were used to amplify a -400-bp genomic fragment 2.1 corresponding to exons 6-8, with an annealing temperature of Pgyl 64~ Allele detection: GreSB and Gre7R amplify a -200-bp Gckr frag- 3.2 ment that, if derived from an M. spretus allele, cuts with XcmI to Cd36 D5Mitl yield fragments of size -150 bp and -50 bp. MboI digestion of the Khk -400-bp genomic fragment produces a fragment of 300 bp 4.3 from the C57BL/6J Khk allele and 220 bp from M. spretus. Previously idenUfied homologs: Human GCKR and KHK co- Dpp6 localize to Chr. 2p23.2-23.3 . 1.1 D5Mit149 Gckr Khk Znt3 Discussion: Gckr encodes the regulatory protein of glucokinase, 1.1 D5Mit351 which binds to and inhibits glucokinase in liver and probably 1.1 Crmp I pancreatic islet [5,6]. This inhibitory interaction is promoted by 3.2 fructose-6-phosphate and relieved by fructose-l-phosphate, the product of ketohexokinase (KHK). The postulated metabolic link Mpmv7 between
Mammalian Genome – Springer Journals
Published: Mar 31, 2009
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.
All for just $49/month
Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.
Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.
It’s easy to organize your research with our built-in tools.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera