Mammalian Genome 8, Brief Data Reports 701 GAAGCCAACC-3') to type the polymorphism by PCR with stan- dard buffers (1.5 rnM MgC12) and an annealing temperature of 55~ For Khk, primers KhkM4 (5'-dTGAGGGGCTTGTA- CAGTCGAG-3') and KhkR9 (5'-dCCACCTGGCACCC- GAATCTC-3'), designed from the sequences of rat  and mouse D5Bir4  Khk cDNA, were used to amplify a -400-bp genomic fragment 2.1 corresponding to exons 6-8, with an annealing temperature of Pgyl 64~ Allele detection: GreSB and Gre7R amplify a -200-bp Gckr frag- 3.2 ment that, if derived from an M. spretus allele, cuts with XcmI to Cd36 D5Mitl yield fragments of size -150 bp and -50 bp. MboI digestion of the Khk -400-bp genomic fragment produces a fragment of 300 bp 4.3 from the C57BL/6J Khk allele and 220 bp from M. spretus. Previously idenUfied homologs: Human GCKR and KHK co- Dpp6 localize to Chr. 2p23.2-23.3 . 1.1 D5Mit149 Gckr Khk Znt3 Discussion: Gckr encodes the regulatory protein of glucokinase, 1.1 D5Mit351 which binds to and inhibits glucokinase in liver and probably 1.1 Crmp I pancreatic islet [5,6]. This inhibitory interaction is promoted by 3.2 fructose-6-phosphate and relieved by fructose-l-phosphate, the product of ketohexokinase (KHK). The postulated metabolic link Mpmv7 between
Mammalian Genome – Springer Journals
Published: Mar 31, 2009
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