Kaiso, a New Protein of the BTB/POZ Family, Specifically Binds to Methylated DNA Sequences

Kaiso, a New Protein of the BTB/POZ Family, Specifically Binds to Methylated DNA Sequences A protein specifically binding to a symmetrically methylated DNA fragment of the first intron of the mts1gene was studied. The protein was purified by gel filtration and affinity chromatography. Mass spectrometry showed that the protein is Kaiso, a new member of the BTB/POZ family. To study the association with methylated DNA sequences in vivo, the location of Kaiso in NIH 3T3 cells was analyzed. Immunofluorescent staining with polyclonal antibodies against Kaiso showed that the protein is predominantly associated with the nucleoli. The causes of its distribution awaits further investigation. The zinc-finger domains of Kaiso were for the first time demonstrated to specifically recognize symmetrically methylated DNA sequences in vitro. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Kaiso, a New Protein of the BTB/POZ Family, Specifically Binds to Methylated DNA Sequences

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Publisher
Springer Journals
Copyright
Copyright © 2001 by MAIK “Nauka/Interperiodica”
Subject
Biomedicine; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1023/A:1016660921580
Publisher site
See Article on Publisher Site

Abstract

A protein specifically binding to a symmetrically methylated DNA fragment of the first intron of the mts1gene was studied. The protein was purified by gel filtration and affinity chromatography. Mass spectrometry showed that the protein is Kaiso, a new member of the BTB/POZ family. To study the association with methylated DNA sequences in vivo, the location of Kaiso in NIH 3T3 cells was analyzed. Immunofluorescent staining with polyclonal antibodies against Kaiso showed that the protein is predominantly associated with the nucleoli. The causes of its distribution awaits further investigation. The zinc-finger domains of Kaiso were for the first time demonstrated to specifically recognize symmetrically methylated DNA sequences in vitro.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Oct 16, 2004

References

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