K+ Channels of Squid Giant Axons Open by an Osmotic Stress in Hypertonic Solutions Containing Nonelectrolytes

K+ Channels of Squid Giant Axons Open by an Osmotic Stress in Hypertonic Solutions Containing... In hypertonic solutions made by adding nonelectrolytes, K+ channels of squid giant axons opened at usual asymmetrical K+ concentrations in two different time courses; an initial instantaneous activation (I IN) and a sigmoidal activation typical of a delayed rectifier K+ channel (I D). The current–voltage relation curve for I IN was fitted well with Goldman equation described with a periaxonal K+ concentration at the membrane potential above −10 mV. Using the activation–voltage curve obtained from tail currents, K+ channels for I IN are confirmed to activate at the membrane potential that is lower by 50 mV than those for I D. Both I IN and I D closed similarly at the holding potential below −100 mV. The logarithm of I IN/I D was linearly related with the osmolarity for various nonelectrolytes. Solute inaccessible volumes obtained from the slope increased with the nonelectrolyte size from 15 to 85 water molecules. K+ channels representing I D were blocked by open channel blocker tetra-butyl ammonium (TBA) more efficiently than in the absence of I IN, which was explained by the mechanism that K+ channels for I D were first converted to those for I IN by the osmotic pressure and then blocked. So K+ channels for I IN were suggested to be derived from the delayed rectifier K+ channels. Therefore, the osmotic pressure is suggested to exert delayed-rectifier K+ channels to open in shrinking rather hydrophilic flexible parts outside the pore than the pore itself, which is compatible with the recent structure of open K+ channel pore. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

K+ Channels of Squid Giant Axons Open by an Osmotic Stress in Hypertonic Solutions Containing Nonelectrolytes

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Publisher
Springer-Verlag
Copyright
Copyright © 2011 by Springer Science+Business Media, LLC
Subject
Life Sciences; Human Physiology; Biochemistry, general
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s00232-011-9383-5
Publisher site
See Article on Publisher Site

Abstract

In hypertonic solutions made by adding nonelectrolytes, K+ channels of squid giant axons opened at usual asymmetrical K+ concentrations in two different time courses; an initial instantaneous activation (I IN) and a sigmoidal activation typical of a delayed rectifier K+ channel (I D). The current–voltage relation curve for I IN was fitted well with Goldman equation described with a periaxonal K+ concentration at the membrane potential above −10 mV. Using the activation–voltage curve obtained from tail currents, K+ channels for I IN are confirmed to activate at the membrane potential that is lower by 50 mV than those for I D. Both I IN and I D closed similarly at the holding potential below −100 mV. The logarithm of I IN/I D was linearly related with the osmolarity for various nonelectrolytes. Solute inaccessible volumes obtained from the slope increased with the nonelectrolyte size from 15 to 85 water molecules. K+ channels representing I D were blocked by open channel blocker tetra-butyl ammonium (TBA) more efficiently than in the absence of I IN, which was explained by the mechanism that K+ channels for I D were first converted to those for I IN by the osmotic pressure and then blocked. So K+ channels for I IN were suggested to be derived from the delayed rectifier K+ channels. Therefore, the osmotic pressure is suggested to exert delayed-rectifier K+ channels to open in shrinking rather hydrophilic flexible parts outside the pore than the pore itself, which is compatible with the recent structure of open K+ channel pore.

Journal

The Journal of Membrane BiologySpringer Journals

Published: Jul 21, 2011

References

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