Populus possesses many characteristics as the model forest species for functional genomic studies, including modest genome size, rapid growth, relative ease of experimental manipulation, and the range of available genetic tools. Isolation of high quality RNA from Populus species and tissues rich in polyphenols and polysaccharides is often difficult. These secondary metabolites not only decrease the yield, but also the quality of RNA is almost unusable. Here we describe a modified procedure to isolate high quality RNA from Populus various tissues, including roots, green stems, leaves, and buds. Major changes are the additional treatments: SDS extraction and ethanol precipitation after CTAB-LiCl purification. Following this procedure, we routinely obtained the high quality RNA, which was suitable for downstream molecular manipulations. Integrity and purity were evaluated by using gel electrophoresis and spectrophotometry (A260/A230 and A260/A280). A260/A230 ratios were greater than 2.0, and the A260/A280 ratios were always between 1.9 and 2.0. The RNA isolated was successfully used for reverse transcription-polymerase chain reaction, rapid amplification of cDNA ends, and suppression subtraction hybridization library construction.
Russian Journal of Plant Physiology – Springer Journals
Published: Sep 8, 2009
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera