Isolation of grape peroxiredoxin gene responding to abiotic stresses

Isolation of grape peroxiredoxin gene responding to abiotic stresses Peroxiredoxins (Prxs) are peroxidases that reduce hydrogen peroxide (H2O2) and various alkyl hydroperoxides and act as reductants. A full-length cDNA encoding for a Prx polypeptide was isolated and cloned from grape (Vitis vinifera L. cv. Askari) berries. The cDNA was 773 nucleotides in length with a deduced amino acid of 162 residues, possessing one conserved cysteine, which belongs to the type II Prx C. The calculated molecular mass and the predicted isoelectric point of the deduced polypeptide are 17.26 kD and 5.15, respectively. The deduced protein sequence showed a high similarity to PrxII C from other plants, in particular from cotton (Gossypium hirsutum), poplar (Populus trichocarpa), and Citrus sp. The in silico analysis of the promoter region of grape Prx demonstrated the presence of a number of potential cis-acting elements to respond to environmental signals, suggesting that VvPrxII C may respond to a variety of environmental signals, including dehydration, heat, heavy metals, light, pathogens, wounding, and plant hormones. The grape Prx gene was also analyzed for its expressional response to abiotic stress, oxidative stress, and antioxidants application. The results revealed a highly induced response to abiotic stress conditions due to the presence of different putative regulatory elements in its promoter. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Isolation of grape peroxiredoxin gene responding to abiotic stresses

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Publisher
Pleiades Publishing
Copyright
Copyright © 2015 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443715050088
Publisher site
See Article on Publisher Site

Abstract

Peroxiredoxins (Prxs) are peroxidases that reduce hydrogen peroxide (H2O2) and various alkyl hydroperoxides and act as reductants. A full-length cDNA encoding for a Prx polypeptide was isolated and cloned from grape (Vitis vinifera L. cv. Askari) berries. The cDNA was 773 nucleotides in length with a deduced amino acid of 162 residues, possessing one conserved cysteine, which belongs to the type II Prx C. The calculated molecular mass and the predicted isoelectric point of the deduced polypeptide are 17.26 kD and 5.15, respectively. The deduced protein sequence showed a high similarity to PrxII C from other plants, in particular from cotton (Gossypium hirsutum), poplar (Populus trichocarpa), and Citrus sp. The in silico analysis of the promoter region of grape Prx demonstrated the presence of a number of potential cis-acting elements to respond to environmental signals, suggesting that VvPrxII C may respond to a variety of environmental signals, including dehydration, heat, heavy metals, light, pathogens, wounding, and plant hormones. The grape Prx gene was also analyzed for its expressional response to abiotic stress, oxidative stress, and antioxidants application. The results revealed a highly induced response to abiotic stress conditions due to the presence of different putative regulatory elements in its promoter.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Oct 18, 2015

References

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