Arch Virol (2002) 147: 1799–1812
Isolation of differentially expressed genes from white spot virus
(WSV) infected Paciﬁc blue shrimp (Penaeus stylirostris)*
, M. M. Roux
, K. R. Klimpel
, andA. K. Dhar
Division of Comparative Medicine, Massachusetts Institute of Technology,
Cambridge, Massachusetts, U.S.A.
Super Shrimp Inc., National City, California, U.S.A.
Received December 13, 2001; accepted May 8, 2002
Published online July 19, 2002
Summary. To isolate novel cellular factors that are activated or repressed upon
WSV infection, the RNA ﬁngerprints of healthy and WSV infected blue shrimp
(Penaeus stylirostris) were compared using the mRNA differential display
technique. Thirty-two unique differentially expressed, and one constitutively ex-
pressed, cDNA sequences were retrieved. Six of 32 cDNAs showed similarities
with the database entries: cDNA 10G32-142 to a shrimp arginine kinase, 22C48-
201 to shrimp mitochondrial ATPase gene; 22C47-197, 21G49-203 and 20A55-
268 to shrimp ESTs and 20G50-206 to a WSV gene, ORF 116. The constitutively
expressed gene showed signiﬁcant similarity to a yeast elongation factor 1-alpha
gene. The expression of a subset of differentially expressed genes (13 of 32)
was further evaluated by real-time RT-PCR. Ten of 13 genes showed statistically
signiﬁcant changes in expression between healthy and WSV infected animals
suggesting that these genes may play an important role in WSV pathogenesis.
The white spot virus (WSV), the etiologic agent of the white spot disease, is
the most devastating viral pathogen of penaeid shrimp (Penaeus sp.) threaten-
ing the shrimp farming industry worldwide [14, 17]. The virus was ﬁrst re-
ported in East Asia during 1992–1993  and within a few years the virus
spread to the shrimp farming regions of Southeast Asia and North America
. During 1999 and 2000, WSV caused severe losses to the shrimp farming
The sequences reported in this paper has been submitted to the GenBank database under
the accession numbers BQ481394 to BQ481426, and AY117542-AY117544.