Isolation, mutagenesis, and optimization of cultivation conditions of microalgal strains for biodiesel production

Isolation, mutagenesis, and optimization of cultivation conditions of microalgal strains for... Algalogically pure cultures were isolated from different aquatic ecosystems and identified as representatives of green algae, euglena algae, and cyanobacteria. Isolated cultures and collection strains of microalgae were screened for productivity. Induced mutagenesis (exposure to UV radiation, 254 nm, 40 Erg/mm2) and selection yielded a mutant strain C-2m2 of Chlorella pyrenoidosa notable for a high activity of lipid biosynthesis and accumulation. Optimization of its culture conditions was conducted. It was found that the active lipid accumulation by the cells of C. pyrenoidosa strain C-2m2 occurred when nitrogen concentration in the medium was tenfold reduced (to the level of 0.004 g/L) at an illuminance of 4 klx. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Isolation, mutagenesis, and optimization of cultivation conditions of microalgal strains for biodiesel production

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Publisher
Springer Journals
Copyright
Copyright © 2014 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S102144371401018X
Publisher site
See Article on Publisher Site

Abstract

Algalogically pure cultures were isolated from different aquatic ecosystems and identified as representatives of green algae, euglena algae, and cyanobacteria. Isolated cultures and collection strains of microalgae were screened for productivity. Induced mutagenesis (exposure to UV radiation, 254 nm, 40 Erg/mm2) and selection yielded a mutant strain C-2m2 of Chlorella pyrenoidosa notable for a high activity of lipid biosynthesis and accumulation. Optimization of its culture conditions was conducted. It was found that the active lipid accumulation by the cells of C. pyrenoidosa strain C-2m2 occurred when nitrogen concentration in the medium was tenfold reduced (to the level of 0.004 g/L) at an illuminance of 4 klx.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Dec 28, 2013

References

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