Physiol Mol Biol Plants https://doi.org/10.1007/s12298-018-0546-z RESEARCH ARTICLE Isolation and identiﬁcation of a vegetative organ-speciﬁc promoter from maize 1 1 1 • • • HaoQiang Yu Muhammad Hayder Bin Khalid FengZhong Lu 1 1 1 1 1 • • • • FuAi Sun JingTao Qu BingLiang Liu WanChen Li FengLing Fu Received: 3 March 2018 / Revised: 28 April 2018 / Accepted: 3 May 2018 Prof. H.S. Srivastava Foundation for Science and Society 2018 Abstract To avoid the unregulated overexpression of the and 437 bp in length, respectively. These results indicate exogenous genes, speciﬁc or inducible expression is nec- that the promoter of the PPR gene is a GRMZM2G129783 essary for some exogenous genes in transgenic plants. But vegetative organ-speciﬁc promoter and will be useful in little is known about organ- or tissue-speciﬁc promoters in transgenic modiﬁcation of commercial crops for moderate maize. In the present study, the expression of a maize speciﬁc expression after further evaluation in pentatricopeptide repeat (PPR) protein encoding gene, monocotyledons. GRMZM2G129783, was analyzed by RNA-sequencing data and conﬁrmed by quantitative real time PCR. The Keywords Maize Pentatricopeptide repeat protein results showed that the PPR gene speciﬁ- Promoter Vegetative organ speciﬁcity GRMZM2G129783 cally expressed
Physiology and Molecular Biology of Plants – Springer Journals
Published: Jun 4, 2018
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