Isolation and genetic characterization of avian influenza virus H4N6 from ducks in China

Isolation and genetic characterization of avian influenza virus H4N6 from ducks in China An avian influenza virus (AIV) strain belonging to the H4 subtype and provisionally designated as A/duck/China/J1/2012(H4N6) was isolated from diseased ducks with respiratory disease at a commercial poultry farm in Shandong, China, in 2012. The genomic coding sequences of all eight segments of this J1 isolate were determined and used for subsequent analysis. Phylogenetic analysis of all eight segments showed that this duck H4N6 virus was of Eurasian lineage and not American lineage. The results show that the virus probably emerged because of a reassortment event involving other avian H4N6 and H6N1 viruses. Interestingly, this H4N6 virus had all the conserved features common to low-pathogenic AIVs, including the HA cleavage sequence, receptor-binding sequences for the 2,3-linked sialic acid receptor in avian species, and the PB2 627E motif. These results suggest that the duck H4N6 isolate could not cross the species barrier to infect and replicate in mammals, including humans. In addition, screening of the duck serum samples showed that only 0.57 % (2/352) of the individuals had weak but measurable hemagglutination inhibition (HI) antibody titers. The low antibody prevalence data were also supported by the failure to detect H4N6 virus (0/56) in clinical nasal swabs of the ducks. These data indicate an alternate reservoir for the H4N6 virus. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Isolation and genetic characterization of avian influenza virus H4N6 from ducks in China

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Publisher
Springer Vienna
Copyright
Copyright © 2015 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-014-2229-6
Publisher site
See Article on Publisher Site

References

  • Seroepidemiological evidence of avian H4, H5, and H9 influenza A virus transmission to pigs in southeastern China
    Ninomiya, A; Takada, A; Okazaki, K; Shortridge, KF; Kida, H
  • Universal primer set for the full-length amplification of all influenza A viruses
    Hoffmann, E; Stech, J; Guan, Y; Webster, R; Perez, D
  • Structure of the amantadine binding site of influenza M2 proton channels in lipid bilayers
    Cady, SD; Schmidt-Rohr, K; Wang, J; Soto, CS; DeGrado, WF; Hong, M

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