Isolation and functional characterization of a methyl jasmonate-responsive 3-carene synthase from Lavandula x intermedia

Isolation and functional characterization of a methyl jasmonate-responsive 3-carene synthase from... Plant Mol Biol (2017) 93:641–657 DOI 10.1007/s11103-017-0588-6 Isolation and functional characterization of a methyl jasmonate- responsive 3-carene synthase from Lavandula x intermedia 1 1 1 1 Ayelign M. Adal  · Lukman S. Sarker  · Ashley D. Lemke  · Soheil S. Mahmoud   Received: 12 October 2016 / Accepted: 23 January 2017 / Published online: 3 March 2017 © Springer Science+Business Media Dordrecht 2017 Abstract and was up-regulated (1.18- to 3.8-fold) with MeJA 8–72 h Key message A methyl jasmonate responsive 3-carene post-treatment. The results suggest that Li3CARS may have synthase (Li3CARS) gene was isolated from Lavandula a defensive role in Lavandula. x intermedia and functionally characterized in vitro. Abstract Lavenders produce essential oils consisting Keywords 3-Carene synthase · Lavandula · Lavandula mainly of monoterpenes, including the potent antimicro- x intermedia · Methyl jasmonate · Monoterpene synthase · bial and insecticidal monoterpene 3-carene. In this study Transcriptional regulation we isolated and functionally characterized a leaf-specific, methyl jasmonate (MeJA)-responsive monoterpene syn- Abbreviations thase (Li3CARS) from Lavandula x intermedia. The ORF BPPS Bornyl pyrophosphate synthase excluding transit peptides encoded a 64.9 kDa protein that EO Essential oil was expressed in E. coli, and purified with Ni–NTA aga-GPP Geranyl pyrophosphate rose affinity chromatography. The recombinant Li3CARS MeJA Methyl jasmonate converted GPP into 3-carene as the major product, with K mTPS Monoterpene synthase −1 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Isolation and functional characterization of a methyl jasmonate-responsive 3-carene synthase from Lavandula x intermedia

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Publisher
Springer Netherlands
Copyright
Copyright © 2017 by Springer Science+Business Media Dordrecht
Subject
Life Sciences; Plant Sciences; Biochemistry, general; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-017-0588-6
Publisher site
See Article on Publisher Site

Abstract

Plant Mol Biol (2017) 93:641–657 DOI 10.1007/s11103-017-0588-6 Isolation and functional characterization of a methyl jasmonate- responsive 3-carene synthase from Lavandula x intermedia 1 1 1 1 Ayelign M. Adal  · Lukman S. Sarker  · Ashley D. Lemke  · Soheil S. Mahmoud   Received: 12 October 2016 / Accepted: 23 January 2017 / Published online: 3 March 2017 © Springer Science+Business Media Dordrecht 2017 Abstract and was up-regulated (1.18- to 3.8-fold) with MeJA 8–72 h Key message A methyl jasmonate responsive 3-carene post-treatment. The results suggest that Li3CARS may have synthase (Li3CARS) gene was isolated from Lavandula a defensive role in Lavandula. x intermedia and functionally characterized in vitro. Abstract Lavenders produce essential oils consisting Keywords 3-Carene synthase · Lavandula · Lavandula mainly of monoterpenes, including the potent antimicro- x intermedia · Methyl jasmonate · Monoterpene synthase · bial and insecticidal monoterpene 3-carene. In this study Transcriptional regulation we isolated and functionally characterized a leaf-specific, methyl jasmonate (MeJA)-responsive monoterpene syn- Abbreviations thase (Li3CARS) from Lavandula x intermedia. The ORF BPPS Bornyl pyrophosphate synthase excluding transit peptides encoded a 64.9 kDa protein that EO Essential oil was expressed in E. coli, and purified with Ni–NTA aga-GPP Geranyl pyrophosphate rose affinity chromatography. The recombinant Li3CARS MeJA Methyl jasmonate converted GPP into 3-carene as the major product, with K mTPS Monoterpene synthase −1

Journal

Plant Molecular BiologySpringer Journals

Published: Mar 3, 2017

References

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