Plant Mol Biol (2017) 93:641–657 DOI 10.1007/s11103-017-0588-6 Isolation and functional characterization of a methyl jasmonate- responsive 3-carene synthase from Lavandula x intermedia 1 1 1 1 Ayelign M. Adal · Lukman S. Sarker · Ashley D. Lemke · Soheil S. Mahmoud Received: 12 October 2016 / Accepted: 23 January 2017 / Published online: 3 March 2017 © Springer Science+Business Media Dordrecht 2017 Abstract and was up-regulated (1.18- to 3.8-fold) with MeJA 8–72 h Key message A methyl jasmonate responsive 3-carene post-treatment. The results suggest that Li3CARS may have synthase (Li3CARS) gene was isolated from Lavandula a defensive role in Lavandula. x intermedia and functionally characterized in vitro. Abstract Lavenders produce essential oils consisting Keywords 3-Carene synthase · Lavandula · Lavandula mainly of monoterpenes, including the potent antimicro- x intermedia · Methyl jasmonate · Monoterpene synthase · bial and insecticidal monoterpene 3-carene. In this study Transcriptional regulation we isolated and functionally characterized a leaf-specific, methyl jasmonate (MeJA)-responsive monoterpene syn- Abbreviations thase (Li3CARS) from Lavandula x intermedia. The ORF BPPS Bornyl pyrophosphate synthase excluding transit peptides encoded a 64.9 kDa protein that EO Essential oil was expressed in E. coli, and purified with Ni–NTA aga-GPP Geranyl pyrophosphate rose affinity chromatography. The recombinant Li3CARS MeJA Methyl jasmonate converted GPP into 3-carene as the major product, with K mTPS Monoterpene synthase −1
Plant Molecular Biology – Springer Journals
Published: Mar 3, 2017
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera