Isolation and expression analysis of Cu/Zn superoxide dismutase genes from three Caragana species

Isolation and expression analysis of Cu/Zn superoxide dismutase genes from three Caragana species Caragana is a native desert shrub with high forage values and stress tolerance, as well as sand-fixing capabilities. Some Caragana species, including C. korshinskii, C. microphylla, and C. intermedia, are commonly used for vegetation restoration programs in the Loess Plateau region of northwestern China and are known to have ecological benefits and high commercial value. In this study, full-length sequences of Cu/Zn-SOD genes were isolated from three Caragana species using degenerate polymerase chain reaction (PCR) and rapid amplification of cDNA ends (RACE) techniques, and their expression under drought stress conditions were investigated. The cloned SOD cDNAs contained a predicted open reading frame (ORF) of 459 bp encoding a polypeptide of 152 amino acids with a theoretical molecular weight of 15.2 kD. Cu/Zn-SOD cDNA of C. korshinskii and C. intermedia shared 100% sequence identity, implying a close relationship. A 24-bp specific sequence was found in the 3-UTR region of C. microphylla Cu/Zn-SOD cDNA, and reverse transcription RT-PCR and genomic PCR confirmed the feasibility of the 24-bp sequence as a DNA marker for rapid variety identification of C. microphylla. RT-PCR revealed that the expression of the Caragana Cu/Zn SOD genes was induced by PEG-simulated drought stress and ABA. The three Caragana Cu/Zn-SOD genes showed similar expression patterns, and no significant differences in transcriptional level were observed among the three genes. These results increase our understanding of the molecular mechanism of drought tolerance and can be used to improve vegetation restoration programs of Caragana plants. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Isolation and expression analysis of Cu/Zn superoxide dismutase genes from three Caragana species

Loading next page...
 
/lp/springer_journal/isolation-and-expression-analysis-of-cu-zn-superoxide-dismutase-genes-lYageOJZaX
Publisher
Pleiades Publishing
Copyright
Copyright © 2014 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443714050185
Publisher site
See Article on Publisher Site

Abstract

Caragana is a native desert shrub with high forage values and stress tolerance, as well as sand-fixing capabilities. Some Caragana species, including C. korshinskii, C. microphylla, and C. intermedia, are commonly used for vegetation restoration programs in the Loess Plateau region of northwestern China and are known to have ecological benefits and high commercial value. In this study, full-length sequences of Cu/Zn-SOD genes were isolated from three Caragana species using degenerate polymerase chain reaction (PCR) and rapid amplification of cDNA ends (RACE) techniques, and their expression under drought stress conditions were investigated. The cloned SOD cDNAs contained a predicted open reading frame (ORF) of 459 bp encoding a polypeptide of 152 amino acids with a theoretical molecular weight of 15.2 kD. Cu/Zn-SOD cDNA of C. korshinskii and C. intermedia shared 100% sequence identity, implying a close relationship. A 24-bp specific sequence was found in the 3-UTR region of C. microphylla Cu/Zn-SOD cDNA, and reverse transcription RT-PCR and genomic PCR confirmed the feasibility of the 24-bp sequence as a DNA marker for rapid variety identification of C. microphylla. RT-PCR revealed that the expression of the Caragana Cu/Zn SOD genes was induced by PEG-simulated drought stress and ABA. The three Caragana Cu/Zn-SOD genes showed similar expression patterns, and no significant differences in transcriptional level were observed among the three genes. These results increase our understanding of the molecular mechanism of drought tolerance and can be used to improve vegetation restoration programs of Caragana plants.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Aug 21, 2014

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve Freelancer

DeepDyve Pro

Price
FREE
$49/month

$360/year
Save searches from
Google Scholar,
PubMed
Create lists to
organize your research
Export lists, citations
Read DeepDyve articles
Abstract access only
Unlimited access to over
18 million full-text articles
Print
20 pages/month
PDF Discount
20% off