Isolation and characterization of two cyclin-like cDNAs from Arabidopsis

Isolation and characterization of two cyclin-like cDNAs from Arabidopsis Cyclins are key regulators of a family of protein kinases called cyclin-dependent kinases (Cdks). Specific cyclins interact with specific Cdks to regulate the different transition points in the cell cycle. Six mitotic-like cyclins have previously been reported in Arabidopsis thaliana. Using polymerase chain reaction amplified cyclin-box sequences as probes, two new cyclin cDNAs are isolated from Arabidopsis. The deduced amino acid sequences of the isolated cDNAs (Arath;CycB1;3 and Arath;CycB1;4) show the highest sequence similarity with mitotic cyclins. Arath;CycB1;3 is most homologous to the plant CycB1group cyclins and contains a conserved motif that is typical of this group. Arath;CycB1;4, while homologous to Arath;CycB1;2, has some features that make it different from other known mitotic-like cyclins. These data suggest the presence of several distinct cyclins of CycB1 group in Arabidopsis. Analysis of expression of three members of CycB1 group (Arath;CycB1;2, Arath;CycB1;3 and Arath;CycB1;4) in different tissues by reverse transcription-polymerase chain reaction using primers corresponding to unique regions of their cDNAs shows that they are differentially expressed in different tissues. Plant Molecular Biology Springer Journals

Isolation and characterization of two cyclin-like cDNAs from Arabidopsis

Loading next page...
Kluwer Academic Publishers
Copyright © 1998 by Kluwer Academic Publishers
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
Publisher site
See Article on Publisher Site


You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial