Isolation and characterization of fusarium wilt resistance gene analogs in radish

Isolation and characterization of fusarium wilt resistance gene analogs in radish The resistance gene analog (RGA)-based marker strategy is an effective supplement for current marker reservoir of radish disease-resistance breeding. In this study, we identified RGAs based on the conserved nucleotide-binding site (NBS) and S-receptor-like kinase (SRLK) domains. A total of 68 NBS-RGAs and 46 SRLK-RGAs were isolated from two FW-resistant radish inbred lines, B2 and YR31, and one susceptible line, YR15. A BLASTx search revealed that the NBS-RGAs contained six conserved motifs (i.e., P loop, RNBS-A, Kinase-2, RNBS-B, RNBS-C, and GLPL) and the SRLK-RGAs, contained two conserved motifs (i.e., G-type lectin and PAN-AP). A phylogenetic analysis indicated that the NBS-RGAs could be separated into two classes (i.e., toll/interleukin receptor and coiled-coil types), with six subgroups, and the SRLK-RGAs were divided into three subgroups. Moreover, we designed RGA-specific markers from data-mining approach in radish databases. Based on marker analysis, 24 radish inbred lines were clustered into five main groups with a similarity index of 0.44 and showing genetic diversity with resistance variation in those radish inbred lines. The development of RGA-specific primers would be valuable for marker-assisted selection during the breeding of disease-resistant radish cultivars. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png 3 Biotech Springer Journals

Isolation and characterization of fusarium wilt resistance gene analogs in radish

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Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer-Verlag GmbH Germany, part of Springer Nature
Subject
Chemistry; Biotechnology; Agriculture; Cancer Research; Bioinformatics; Stem Cells; Biomaterials
ISSN
2190-572X
eISSN
2190-5738
D.O.I.
10.1007/s13205-018-1279-y
Publisher site
See Article on Publisher Site

Abstract

The resistance gene analog (RGA)-based marker strategy is an effective supplement for current marker reservoir of radish disease-resistance breeding. In this study, we identified RGAs based on the conserved nucleotide-binding site (NBS) and S-receptor-like kinase (SRLK) domains. A total of 68 NBS-RGAs and 46 SRLK-RGAs were isolated from two FW-resistant radish inbred lines, B2 and YR31, and one susceptible line, YR15. A BLASTx search revealed that the NBS-RGAs contained six conserved motifs (i.e., P loop, RNBS-A, Kinase-2, RNBS-B, RNBS-C, and GLPL) and the SRLK-RGAs, contained two conserved motifs (i.e., G-type lectin and PAN-AP). A phylogenetic analysis indicated that the NBS-RGAs could be separated into two classes (i.e., toll/interleukin receptor and coiled-coil types), with six subgroups, and the SRLK-RGAs were divided into three subgroups. Moreover, we designed RGA-specific markers from data-mining approach in radish databases. Based on marker analysis, 24 radish inbred lines were clustered into five main groups with a similarity index of 0.44 and showing genetic diversity with resistance variation in those radish inbred lines. The development of RGA-specific primers would be valuable for marker-assisted selection during the breeding of disease-resistant radish cultivars.

Journal

3 Biotech – Springer Journals

Published: May 14, 2018

References

  • Different requirements for EDS1 and NDR1 by disease resistance genes define at least two R gene-mediated signaling pathways in Arabidopsis
    Aarts, N; Metz, M; Holub, E; Staskawicz, BJ; Daniels, MJ; Parker, JE
  • The barley stem rust-resistance gene Rpg1 is a novel disease-resistance gene with homology to receptor kinases
    Brueggeman, R; Rostoks, N; Kudrna, D; Kilian, A; Han, F; Chen, J; Druka, A; Steffenson, B; Kleinhofs, A
  • The tomato I-3 gene: a novel gene for resistance to Fusarium wilt disease
    Catanzariti, AM; Lim, GT; Jones, DA
  • Diversity in receptor-like kinase genes is a major determinant of quantitative resistance to Fusarium oxysporum f.sp. matthioli
    Cole, SJ; Diener, AC
  • Cloning and characterization of NBS-LRR class resistance-gene candidate sequences in citrus
    Deng, Z; Huang, S; Ling, P; Chen, C; Yu, C; Weber, CA; Moore, GA; Gmitter, FG
  • Resistance to Fusarium oxysporum 1, a dominant Arabidopsis disease-resistance gene, is not race specific
    Diener, AC; Ausubel, FM
  • Activation, structure, and organization of genes involved in microbial defense in plants
    Dixon, RA; Harrison, MJ
  • A superfamily of S locus-related sequences in Arabidopsis: diverse structures and expression patterns
    Dwyer, KG; Kandasamy, MK; Mahosky, DI; Acciai, J; Kudish, BI; Miller, JE; Nasrallah, ME; Nasrallah, JB
  • Structure, function and evolution of plant disease resistance genes
    Ellis, J; Dodds, P; Pryor, T
  • Evolutionary trees from DNA sequences: a maximum likelihood approach
    Felsenstein, J
  • Identification of I-7 expands the repertoire of genes for resistance to Fusarium wilt in tomato to three resistance gene classes
    Gonzalez-Cendales, Y; Catanzariti, AM; Baker, B; McGrath, DJ; Jones, DA
  • Protein kinases 6. The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification
    Hanks, SK; Hunter, T
  • Cloning, characterization, and evolution of the NBS-LRR-encoding resistance gene analogue family in polyploid cotton (Gossypium hirsutum L.)
    He, L; Du, C; Covaleda, L; Xu, Z; Robinson, AF; Yu, JZ; Kohel, RJ; Zhang, HB
  • The role of leucine-rich repeat proteins in plant defences
    Jones, DA; Jones, JDG
  • Mapping and analysis of a novel candidate Fusarium wilt resistance gene FOC1 in Brassica oleracea
    Lv, H; Fang, Z; Yang, L; Zhang, Y; Wang, Q; Liu, Y; Zhuang, M; Yang, Y; Xie, B; Liu, B; Liu, J; Kang, J; Wang, X
  • Plant NBS-LRR proteins: adaptable guards
    McHale, L; Tan, X; Koehl, P; Michelmore, RW
  • Salicylic acid induces the expression of a number of receptor-like kinase genes in Arabidopsis thaliana
    Ohtake, Y; Takahashi, T; Komeda, Y
  • Divergent evolution of plant NBS-LRR resistance gene homologues in dicot and cereal genomes
    Pan, Q; Wendel, J; Fluhr, R
  • Comparison of the expression patterns of two small gene families of S gene family receptor kinase genes during the defence response in Brassica oleracea and Arabidopsis thaliana
    Pastuglia, M; Swarup, R; Rocher, A; Saindrenan, P; Roby, D; Dumas, C; Cock, JM
  • Resistance gene analogues as a tool for rapid identification and cloning of disease resistance genes in plants—a review
    Sharma, TR; Das, A; Kumar, SP; Lodha, ML
  • Arabidopsis thaliana resistance to Fusarium oxysporum 2 implicates tyrosine-sulfated peptide signaling in susceptibility and resistance to root infection
    Shen, Y; Diener, AC
  • Identification of candidate genes for Fusarium yellows resistance in Chinese cabbage by differential expression analysis
    Shimizu, M; Fujimoto, R; Ying, H; Pu, ZJ; Ebe, Y; Kawanabe, T; Saeki, N; Taylor, JM; Kaji, M; Dennis, ES; Okazaki, K
  • Dissection of the fusarium I2 gene cluster in tomato reveals six homologs and one active gene copy
    Simons, G; Groenendijk, J; Wijbrandi, J; Reijans, M; Groenen, J; Diergaarde, P; Lee, T; Bleeker, M; Onstenk, J; Both, M; Haring, M; Mes, J; Cornelissen, B; Zabeau, M; Vos, P
  • An Arabidopsis thaliana gene with sequence similarity to the s-locus receptor kinase of Brassica oleracea: sequence and expression
    Tobias, CM; Howlett, B; Nasrallah, JB
  • Molecular markers and plant biotechnology
    Tomar, RS; Parakhia, MV; Patel, SV; Golakiya, BA
  • Analysis of TIR- and non-TIR-NBS-LRR disease resistance gene analogous in pepper: characterization, genetic variation, functional divergence and expression patterns
    Wan, H; Yuan, W; Ye, Q; Wang, R; Ruan, M; Li, Z; Zhou, G; Yao, Z; Zhao, J; Liu, S; Yang, Y
  • Resistance gene analogue isolation and RGA-based marker development for identifying downy mildew resistance in radish (Raphanus sativus L.)
    Wang, XL; Xu, L; Song, ZH; Zhu, XW; Wang, Y; Wang, RH; Gong, YQ; Limera, C; Liu, LW
  • Identification of three putative signal transduction genes involved in R gene-specified disease resistance in Arabidopsis
    Warren, RF; Merritt, PM; Holub, E; Innes, RW
  • The I2 resistance gene homologues in Solanum have complex evolutionary patterns and are targeted by miRNAs
    Wei, CH; Kuang, HH; Li, F; Chen, JJ
  • Isolation of a family of resistance gene analogue sequences of the nucleotide binding site (NBS) type from Lens species
    Yaish, MW; Saenz de Miera, LE; PerezVega, M
  • Comparative mapping of Raphanus sativus genome using Brassica markers and quantitative trait loci analysis for the Fusarium wilt resistance trait
    Yu, X; Choi, SR; Ramchiary, N; Miao, X; Lee, SH; Sun, HJ; Kim, S; Ahn, CH; Lim, YP

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