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- Publisher
- Springer Journals
- Copyright
- Copyright © 2004 by Kluwer Academic Publishers
- Subject
- Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
- ISSN
- 0167-4412
- eISSN
- 1573-5028
- DOI
- 10.1023/B:PLAN.0000036366.57794.ee
- pmid
- 15284491
- Publisher site
- See Article on Publisher Site
Abstract
The liverwort Marchantia polymorpha contains high proportions of arachidonic and eicosapentaenoic acids. In general, these C20 polyunsaturated fatty acids (PUFA) are synthesized from linoleic and α-linolenic acids, respectively, by a series of reactions catalyzed by Δ6-desaturase, an ELO-like enzyme involved in Δ6 elongation and Δ5-desaturase. Here we report the isolation and characterization of the cDNAs, MpDES6, MpELO1 and MpDES5, coding for the respective enzymes from M. polymorpha. Co-expression of the MpDES6, MpELO1 and MpDES5 cDNAs resulted in the accumulation of arachidonic and eicosapentaenoic acids in the methylotrophic yeast Pichia pastoris. Interestingly, Δ6 desaturation by the expression of the MpDES6 cDNA appears to occur both in glycerolipids and the acyl-CoA pool, although other lower-plant Δ6-desaturases are known to have a strong preference for glycerolipids.
Journal
Plant Molecular Biology – Springer Journals
Published: Oct 10, 2004