Isolation and characterisation of tobacco (Nicotiana tabacum) cDNA clones encoding proteins involved in magnesium chelation into protoporphyrin IX

Isolation and characterisation of tobacco (Nicotiana tabacum) cDNA clones encoding proteins... We have identified cDNA clones encoding the two Mg chelatase subunits CHL I and CHL H from tobacco (Nicotiana tabacum) by screening a cDNA library with homologous cDNA fragments from Arabidopsis thaliana. A full-length Chl I cDNA clone encodes a peptide with 426 amino acids. The entire cDNA sequence encoding 1382 amino acid long CHL H was obtained by extension of a truncated cDNA fragment using the ‘rapid amplification of cDNA ends’ (RACE) method. Both genes Chl I and Chl H were strongly expressed in young leaves and to a lesser extent in mature leaves. Only traces of both transcripts were found in flowering organs. Southern blot analysis suggests that CHL I is encoded by a single gene and CHL H most likely by several genes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Isolation and characterisation of tobacco (Nicotiana tabacum) cDNA clones encoding proteins involved in magnesium chelation into protoporphyrin IX

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Publisher
Springer Journals
Copyright
Copyright © 1997 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1005913315905
Publisher site
See Article on Publisher Site

Abstract

We have identified cDNA clones encoding the two Mg chelatase subunits CHL I and CHL H from tobacco (Nicotiana tabacum) by screening a cDNA library with homologous cDNA fragments from Arabidopsis thaliana. A full-length Chl I cDNA clone encodes a peptide with 426 amino acids. The entire cDNA sequence encoding 1382 amino acid long CHL H was obtained by extension of a truncated cDNA fragment using the ‘rapid amplification of cDNA ends’ (RACE) method. Both genes Chl I and Chl H were strongly expressed in young leaves and to a lesser extent in mature leaves. Only traces of both transcripts were found in flowering organs. Southern blot analysis suggests that CHL I is encoded by a single gene and CHL H most likely by several genes.

Journal

Plant Molecular BiologySpringer Journals

Published: Sep 30, 2004

References

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