ISSN 10214437, Russian Journal of Plant Physiology, 2015, Vol. 62, No. 3, pp. 420–423. © Pleiades Publishing, Ltd., 2015.
Original Russian Text © I.M. Andreev, 2015, published in Fiziologiya Rastenii, 2015, Vol. 62, No. 3, pp. 452–455.
In 2006 in “Russian Journal of Plant Physiology”
the article “Ionophorous functions of phosphatidic
acid in the plant cell” by Medvedev et al.  was pub
lished. The results of the experiments carried out in
this work demonstrated, by author opinion, an ability
of phosphatidic acid (PA) to transfer Ca
through plant cell membranes. This article leads to a
whole number of questions because at present nobody
says about such properties of PA. Nevertheless,
authors of this publication continue so far their studies
in this direction. In particular, this follows from their
short report “Membrane mechanism of the function
ing of phosphatidic acids as second messengers of the
plant cell” recently published in materials of the
Annual Meeting of Russian Plant Physiologist Society
“Plant physiology is the theoretical base for innova
tional agrarian and phytobiotechnologies” (Kalinin
grad, 2014, part I, pp. 84–85). Moreover, here, it was
noted that these studies were financially supported by
the grant of Russian Foundation for Basic Research.
In this connection, it is still early to say that time
already has put everything in its place, and there is a
need to clarify the problem in question. Discussion of
several important aspects of the latter is the purpose of
the present work.
Published in the author’s version.
To date, it was convincingly proved that PA belongs
to a number of the second messengers participating in
transduction of intracellular signals in eukaryotic cells.
It is known that PA is relatively simple anionic lipid
functioning in the role of a key intermediate in the
biosynthesis of neutral lipids and all glycerophospho
lipids [2–4]. The activity of PA as a second messenger
is expressed in triggering, modulation, and regulation
of various cellular processes including the vesicle
transport, cytoskeleton activity dynamics, and also
cell responses to the action of different stress factors.
The concentration of PA in cells of yeast and animal
organisms is kept at low enough level and amounts
only 1% in relative to all content of lipids in them that
is a result of its constant conversion into other lipid
types being supported by its de novo synthesis. How
ever, in plant cells this value may be achieved 5–10%
. At the same time, it is known that in many cases
the signaling function of PA is initiated after produc
tion of this lipid by hydrolysis of phosphatidylcholine
under the action of phospholipase D or phosphoryla
tion of diacylglycerine by diacylglycerine kinase .
Although the mechanism underlying the signal
transduction with participation of PA remains in great
deal so far unclear the available literature data show
that to date a significant progress in our understanding
a number of particularities concerning realization of
its signaling function was already achieved. As recently
clarified, these are largely determined by special phys
TO THE EDITOR
Is the Ionophorous Action Inherent in Phosphatidic Acid as a Possible
Basis for Its Mediator Function in Intracellular
I. M. Andreev
Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya ul. 35, Moscow, 127276 Russia;
fax: +7 (499) 9778018; email: email@example.com
Received September 19, 2014
—This work is critical analysis of the literature data providing, by author opinion, evidence for an
ability of phosphatidic acid (PA) to facilitated transfer of Ca
ions through plant cell membranes as
a possible basis for realization of its known mediator function in intracellular signal transduction. Taking into
account a number of arguments author of the present work led to the conclusion that the data under consid
eration do not provide sufficient grounds to believe that PA is capable of carrying out the ionophorous func
tion. In addition it was shown that the opinion of supporters of the “PA ionophorous function” concept is not
in accordance with many results of other studies arguing in favor of the interaction of PA with specific effector
proteins including the cell membrane ion channels catalyzing transmembrane transport of Ca
cations and thereby being responsible for calcium signal generation, the effect as known capable of being
induced by PA.
: plants, cell membranes, phosphatidic acid, calcium, indo1, chlortetracycline, ion transport
: PA—phosphatidic acid.